Improvement of culture conditions of human embryoid bodies using a controlled perfused and dialyzed bioreactor system

Tissue Engineering. Part C, Methods
Julien CômeMichel Cailleret

Abstract

In parallel to the active search for therapeutic and industrial applications of human embryonic stem cells (hESCs), designing automated means of producing those cells is a timely goal. Slow-turning lateral vessels (STLVs) with low shear stress have shown promise for expanding the cells at the embryoid body stage. We have improved this technology by developing two complementary systems, allowing continuous optimization of the culture conditions. First, perfused STLV bioreactors were set up, to provide continuous delivery of culture medium to the cells growing in the rotating chamber. This allowed the external control of the culture medium, and consequently optimized oxygenation, pH, nutrient supply, and waste elimination. Second, a dialysis chamber was adapted. This led to a further enhanced controlled environment and a decrease in the quantity of adjunct products (e.g., growth factors) necessary to the cells inside the bioreactor chamber. hESC aggregation and initial differentiation-taking neural induction as an example-were compared between the perfused and dialyzed STLV system and static cultures. Perfused and dialyzed STLV bioreactors promoted formation of embryoid bodies that were differentiated more rapidly and were homoge...Continue Reading

References

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Citations

Apr 16, 2011·Tissue Engineering. Part B, Reviews·Melissa A KinneyTodd C McDevitt
Apr 23, 2009·Tissue Engineering. Part C, Methods·Jörg C GerlachKatrin Zeilinger
Sep 24, 2009·BMC Biotechnology·Margarida SerraPaula M Alves
Dec 31, 2009·World Journal of Stem Cells·Sasitorn RungarunlertAndras Dinnyes
May 1, 2012·Trends in Biotechnology·Margarida SerraPaula M Alves
Feb 18, 2010·Biochemical Engineering Journal·Samira M Azarin, Sean P Palecek
Apr 8, 2011·Stem Cells·Gilles LemaîtreMarc Peschanski
Apr 23, 2015·Stem Cells and Development·Giuseppe PettinatoNing Zhang
Jul 6, 2011·Biotechnology Advances·Carlos A V RodriguesJoaquim M S Cabral
Dec 24, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Otto-Wilhelm Merten
Jul 28, 2010·Biochemical Society Transactions·Delphine LaustriatMarc Peschanski
Aug 28, 2020·Frontiers in Bioengineering and Biotechnology·Michelle A M VisSandra Hofmann

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Methods Mentioned

BETA
electrophoresis
fluorescence-activated cell sorting
FACS
flow cytometry
PCR

Software Mentioned

- it
CellQuest
Analyse
- it General Statistics
Opticon Monitor

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