Improvement of efficiency in the enzymatic synthesis of lactulose palmitate

Journal of Agricultural and Food Chemistry
Claudia BernalLorena Wilson

Abstract

Sugar esters are considered as surfactants due to its amphiphilic balance that can lower the surface tension in oil/water mixtures. Enzymatic syntheses of these compounds are interesting both from economic and environmental considerations. A study was carried out to evaluate the effect of four solvents, temperature, substrate molar ratio, biocatalyst source, and immobilization methodology on the yield and specific productivity of lactulose palmitate monoester synthesis. Lipases from Pseudomonas stutzeri (PsL) and Alcaligenes sp. (AsL), immobilized in porous silica functionalized with octyl groups (adsorption immobilization, OS) and with glyoxyl-octyl groups (both adsorption and covalent immobilization, OGS), were used. The highest lactulose palmitate yields were obtained at 47 °C in acetone, for all biocatalysts, while the best lactulose:palmitic acid molar ratio differed according to the immobilization methodology, being 1:1 for AsL-OGS biocatalyst (20.7 ± 3%) and 1:3 for the others (30-50%).

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Citations

May 20, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nazzoly RuedaRoberto Fernandez-Lafuente
Feb 2, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Vinicius VescoviPaulo W Tardioli
Sep 8, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Carolina TufiñoLorena Wilson
Sep 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jose J Virgen-OrtízRoberto Fernandez-Lafuente
Sep 1, 2016·Comprehensive Reviews in Food Science and Food Safety·Sara C SilvérioLígia R Rodrigues
Aug 30, 2021·Biotechnology Advances·Rafael C RodriguesRoberto Fernandez-Lafuente

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