Improvement of enzymatic saccharification yield in Arabidopsis thaliana by ectopic expression of the rice SUB1A-1 transcription factor

PeerJ
Lizeth Núñez-LópezJulián M Peña-Castro

Abstract

Saccharification of polysaccharides releases monosaccharides that can be used by ethanol-producing microorganisms in biofuel production. To improve plant biomass as a raw material for saccharification, factors controlling the accumulation and structure of carbohydrates must be identified. Rice SUB1A-1 is a transcription factor that represses the turnover of starch and postpones energy-consuming growth processes under submergence stress. Arabidopsis was employed to test if heterologous expression of SUB1A-1 or SUB1C-1 (a related gene) can be used to improve saccharification. Cellulolytic and amylolytic enzymatic treatments confirmed that SUB1A-1 transgenics had better saccharification yield than wild-type (Col-0), mainly from accumulated starch. This improved saccharification yield was developmentally controlled; when compared to Col-0, young transgenic vegetative plants yielded 200-300% more glucose, adult vegetative plants yielded 40-90% more glucose and plants in reproductive stage had no difference in yield. We measured photosynthetic parameters, starch granule microstructure, and transcript abundance of genes involved in starch degradation (SEX4, GWD1), juvenile transition (SPL3-5) and meristematic identity (FUL, SOC1) but ...Continue Reading

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Citations

Mar 5, 2016·The FEBS Journal·David A MeekinsMatthew S Gentry
Mar 21, 2020·Frontiers in Plant Science·Francesco Pancaldi, Luisa M Trindade
Sep 28, 2017·BioMed Research International·Julián Mario Peña-CastroLorena Amaya-Delgado

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Datasets Mentioned

BETA
GSE27669

Methods Mentioned

BETA
transgenics
transgenic
scanning electron microscopy
PCR

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