Improvement of enzyme-linked immunosorbent assay for the multicolor detection of biomarkers

Chemical Science
Chao LiGenxi Li

Abstract

An enzyme-linked immunosorbent assay that is dependent on enzyme amplification has dominated the current field of protein detection; however, limited multiple detection ability and susceptible enzymatic reactions, and low sensitivity may severely hinder its application. Here, we report a new signal amplification scheme based on allochroic molecule modified carboxyl graphene oxide (cGO), which can be used to develop a multicolor immunoassay named as allochroic-cGO linked immunosorbent assay (ALISA). Thanks to high adsorption levels and a wide selection of allochroic molecules, the simultaneous colorimetric detection of diagnostic biomarkers at a picogram level can be successfully achieved for the first time. In addition, the color change triggered by acidic or basic water can provide a simple, rapid, stable and economical signal output, further meeting the growing biodetection requirements. Moreover, with the help of ALISA, we demonstrate that the combined detection of three tumor biomarkers, including carcino-embryonic antigen, neuron-specific enolase, and cytokeratin-19 fragment, is more valuable for differentiating lung cancer patients than the detection of a single biomarker, further manifesting the superiority of ALISA. All...Continue Reading

References

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Citations

May 22, 2020·The Analyst·Rabbee G MahmudunnabiMuhammad J A Shiddiky
Mar 29, 2020·The Analyst·Lei HuangJinhong Guo
May 15, 2021·Biosensors & Bioelectronics·Jiantao PingQiongzheng Hu
Jun 18, 2021·Advanced Materials·Xinyi LiuWanwan Li
May 31, 2019·Analytical Chemistry·Lei JiaoChengzhou Zhu
Oct 11, 2019·Analytical Chemistry·Li-Peng SunBai-Ou Guan
Apr 12, 2019·Analytical Chemistry·Zhian HuXinrong Zhang
Jan 5, 2022·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yujing ZengGenxi Li

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
biosensing
X-ray
amidation
infrared spectroscopy
atomic force microscopy
ALISA

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