PMID: 8471157Apr 1, 1993Paper

Improvement of human keratinocyte isolation and culture using thermolysin

Burns : Journal of the International Society for Burn Injuries
L GermainF A Auger

Abstract

We propose a modification of the conventional keratinocyte isolation method which has shown a significant improvement in the purity, colony forming efficiency (c.f.e.) and growth capacity of the isolated epidermal cell population. This method utilized thermolysin since it selectively digests the dermo-epidermal junction. Following separation from the dermis, the epidermis was digested with trypsin to obtain a single cell suspension. Compared with the conventional procedure, this isolation method was shorter and resulted in (i) cells displaying a higher colony forming efficiency, (ii) cells reaching confluence 1-3 days earlier, (iii) cells not contaminated by fibroblasts, (iv) a cell population containing all the basal layer keratinocytes. These cells were suitable for the establishment of primary cultures and could be subcultured. Such cell populations should be advantageous in studies of epithelial-mesenchymal interactions in which keratinocyte populations, free of fibroblasts, are desirable. In the treatment of extensively burned patients using cultured epidermal sheets, the main problem remains the time required for their production. Thus, the absence of fibroblast overgrowth of the keratinocyte cultures and the significantl...Continue Reading

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Citations

May 23, 2000·Microscopy Research and Technique·L P PageotJ F Beaulieu
Jun 1, 2000·Medical & Biological Engineering & Computing·L GermainF Auger
Nov 1, 1993·In Vitro Cellular & Developmental Biology. Animal·M MichelL Germain
Jun 1, 1995·In Vitro Cellular & Developmental Biology. Animal·F A AugerL Germain
Aug 27, 2013·Archives of Dermatological Research·Simon GuérardPatrice E Poubelle
Jul 14, 2012·Clinical & Experimental Metastasis·Laure GibotFrançois A Auger
Oct 16, 2008·In Vitro Cellular & Developmental Biology. Animal·Bisera CvetkovskaLucie Germain
Nov 17, 2009·In Vitro Cellular & Developmental Biology. Animal·Somayeh ReiisiAbolfazl Shirazi
Sep 7, 1999·In Vitro Cellular & Developmental Biology. Animal·M MichelL Germain
Mar 1, 1996·Burns : Journal of the International Society for Burn Injuries·F GouletF A Auger
May 1, 1996·Burns : Journal of the International Society for Burn Injuries·W XuL Germain
May 1, 1995·Burns : Journal of the International Society for Burn Injuries·L GermainF A Auger
Dec 16, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Sébastien GingrasJacques Simard
Aug 6, 2003·Life Sciences·Agnese FerrettiFranco Mosca
May 28, 1998·Burns : Journal of the International Society for Burn Injuries·K A WrightB M Wentworth
Nov 3, 2001·Matrix Biology : Journal of the International Society for Matrix Biology·F BerthodF A Auger
Aug 5, 2000·Progress in Retinal and Eye Research·L GermainS L Guérin
Apr 12, 2000·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Z Ruszczak, R A Schwartz
Jan 1, 1996·Tissue Engineering·S T Boyce
Jul 7, 2005·Tissue Engineering·Annie F BlackOdile Damour
Oct 19, 2007·Tissue Engineering·Gregor BoeseAndreas Lendlein
Dec 18, 2009·Tissue Engineering. Part a·Sara BouhoutStéphane Bolduc
Nov 12, 2010·Tissue Engineering. Part a·Jessica JeanRoxane Pouliot
Feb 4, 2011·Tissue Engineering. Part a·Gabrielle OuelletStéphane Bolduc
Mar 23, 2011·Tissue Engineering. Part a·Jessica JeanRoxane Pouliot
Nov 18, 2009·Tissue Engineering. Part C, Methods·Nadia ZakariaMarie-José Tassignon
Nov 1, 2011·Human Molecular Genetics·Richard JennemannRoger Sandhoff
Aug 15, 1996·Transplantation·C A López ValleF A Auger
Jun 27, 2002·Transplantation·Roxane PouliotLucie Germain
Mar 15, 2005·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Howard FeinPhilip L Bailin
Nov 1, 1998·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·H LafranceF A Auger

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