Improvement of inhibitor tolerance in Saccharomyces cerevisiae by overexpression of the quinone oxidoreductase family gene YCR102C

FEMS Yeast Research
Hongqi ChenXinqing Zhao

Abstract

Budding yeast Saccharomyces cerevisiae is widely used for lignocellulosic biorefinery. However, its fermentation efficiency is challenged by various inhibitors (e.g. weak acids, furfural) in the lignocellulosic hydrolysate, and acetic acid is commonly present as a major inhibitor. The effects of oxidoreductases on the inhibitor tolerance of S. cerevisiae have mainly focused on furfural and vanillin, whereas the influence of quinone oxidoreductase on acetic acid tolerance is still unknown. In this study, we show that overexpression of a quinone oxidoreductase-encoding gene, YCR102C, in S. cerevisiae, significantly enhanced ethanol production under acetic acid stress as well as in the inhibitor mixture, and also improved resistance to simultaneous stress of 40°C and 3.6 g/L acetic acid. Increased catalase activities, NADH/NAD+ ratio and contents of several metals, especially potassium, were observed by YCR102C overexpression under acetic acid stress. To our knowledge, this is the first report that the quinone oxidoreductase family protein is related to acid stress tolerance. Our study provides a novel strategy to increase lignocellulosic biorefinery efficiency using yeast cell factory.

References

Aug 6, 2003·European Journal of Biochemistry·Héctor Riveros-RosasEnrique Piña
Dec 27, 2005·Bioscience, Biotechnology, and Biochemistry·Emiko KitagawaHitoshi Iwahashi
May 15, 2007·Cellular and Molecular Life Sciences : CMLS·M R FernándezX Parés
Oct 27, 2010·Microbial Cell Factories·Nuno P MiraIsabel Sá-Correia
Sep 10, 2013·Methods in Enzymology·Megan Bergkessel, Christine Guthrie
May 6, 2014·Trends in Biotechnology·Xiulai ChenLiming Liu
Oct 4, 2014·Science·Felix H LamGregory Stephanopoulos
Dec 1, 2015·Frontiers in Bioengineering and Biotechnology·Luis CaspetaJens Nielsen
Mar 8, 2017·Applied Microbiology and Biotechnology·Han-Yu WangMeng -Gen Ma
Jun 7, 2017·FEMS Yeast Research·Quinten DeparisJohan M Thevelein
Jun 26, 2017·Microbial Cell Factories·Chunhua ZhaoYanping Zhang
Oct 11, 2017·Bioresource Technology·Goldy De BhowmickRamkrishna Sen
Nov 7, 2018·Applied Microbiology and Biotechnology·Joana T CunhaLucília Domingues
Jan 8, 2019·Journal of Biotechnology·Eun Joong OhYong-Su Jin

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