Improvement of L-ornithine production by attenuation of argF in engineered Corynebacterium glutamicum S9114

AMB Express
Bin ZhangBang-Ce Ye

Abstract

L-Ornithine, a non-essential amino acid, has enormous industrial applications in food, pharmaceutical, and chemical industries. Currently, L-ornithine production is focused on microorganism fermentation using Escherichia coli or Corynebacterium glutamicum. In C. glutamicum, development of high L-ornithine producing C. glutamicum was achieved by deletion of argF, but was accompanied by growth deficiency and arginine auxotrophy. L-Arginine has been routinely added to solve this problem; however, this increases production cost and causes feedback inhibition of N-acetyl-L-glutamate kinase activity. To avoid the drawbacks of growth disturbance due to disruption of ArgF, strategies were adopted to attenuate its expression. Firstly, ribosome binding site substitution and start codon replacement were introduced to construct recombinant C. glutamiucm strains, which resulted in an undesirable L-ornithine production titer. Then, we inserted a terminator (rrnB) between argD and argF, which significantly improved L-ornithine production and relieved growth disturbance. Transcription analysis confirmed that a terminator can be used to downregulate expression of argF and simultaneously improve the transcriptional level of genes in front of arg...Continue Reading

References

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Citations

Nov 13, 2018·Frontiers in Microbiology·Min Ju Lee, Pil Kim
Mar 18, 2020·Chembiochem : a European Journal of Chemical Biology·Zhiyun HeZhenghong Xu
Jun 15, 2020·Journal of Industrial Microbiology & Biotechnology·Ying-Yu WangWei-Guo Zhang
Jan 31, 2020·Frontiers in Bioengineering and Biotechnology·Xiao-Yu WuBang-Ce Ye
Dec 5, 2018·Metabolic Engineering·Abigail N LeistraLydia M Contreras
Aug 28, 2021·International Journal of Molecular Sciences·Ning LiuJian-Zhong Xu

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Methods Mentioned

BETA
targeted modifications
PCR
gene knockout

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