Improvement of oocyte in vitro maturation from mice with polycystic ovary syndrome by human mesenchymal stromal cell-conditioned media

Journal of Cellular Biochemistry
Hamideh JafarzadehAbbas Piryaei

Abstract

The outcome of in vitro maturation (IVM) in the patients with polycystic ovary syndrome (PCOS) is poor. Abnormal intraovarian paracrine interplay alters microenvironment for oocyte development through folliculogenesis and decreases developmental competence of oocytes in patients with PCOS. Mesenchymal stromal cells (MSCs) secrete a variety of cytokines and growth factors that could promote oocyte maturation in vitro. Thus, in the current study we aimed to evaluate the effect of human bone marrow MSC-conditioned media (hBM-MSC-CM), as a supplement, to enrich IVM medium for PCOS germinal vesicles (GVs). For this purpose, oocytes at GV and metaphase II (MII) stages were harvested from PCOS mice. The GVs were randomly divided into four groups and incubated for 24 hours in an IVM medium (TCM199, as the control group) or TCM199 supplemented by 25%, 50%, and 75% of hBM-MSC-CM (PCOS-CM25, PCOS-CM50, and PCOS-CM75 groups, respectively) so as to evaluate which dose(s) could enhance maturation rate of the GVs and their subsequent in vitro fertilization (IVF) outcome. Furthermore, MII oocytes and their subsequent IVF outcome were considered as the in vivo matured (PCOS-IVO) group. The data showed that supplementation of IVM medium with 50%...Continue Reading

References

Mar 1, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·M SakaguchiN L First
Aug 17, 2004·Molecular Endocrinology·Erik JansenBart C J M Fauser
Aug 27, 2005·Human Reproduction Update·E M E W HeijnenB C J M Fauser
Feb 28, 2006·Human Reproduction·Thomas TangAdam H Balen
May 8, 2007·Fertility and Sterility·Byung Chul JeeUNKNOWN Seoul National University College of Medicine Assisted Reproductive Technology (SMART) Study Group
Dec 18, 2007·Obstetrical & Gynecological Survey·Daniel A DumesicDavid H Abbott
May 24, 2008·Human Reproduction Update·Stephen FranksKate Hardy
Sep 3, 2008·Placenta·F Ubaldi, L Rienzi
Nov 29, 2008·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·B LingZ G Tian
May 19, 2009·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·D Q FengZ G Tian
Aug 19, 2009·Stem Cells and Development·Byung Sun YoonSeungkwon You
Mar 2, 2010·The Journal of Steroid Biochemistry and Molecular Biology·A B Motta
Jul 2, 2010·Reproduction, Fertility, and Development·Li-Ying YanPing Liu
Sep 30, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Kun ZhangAlan D Ealy
Jul 24, 2012·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Einat Shalom-PazBenny Almog
Apr 23, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Xin HuangLili Chen
Feb 8, 2014·Clinical and Experimental Reproductive Medicine·Kyung Sil LimWon Don Lee
Jul 22, 2014·Clinical and Experimental Reproductive Medicine·Eun Mi ChangTae Ki Yoon
Aug 18, 2016·Animal Science Journal = Nihon Chikusan Gakkaihō·Fatemeh NikmardReza Aflatoonian
Apr 27, 2017·Reproduction, Fertility, and Development·Fatemeh EiniArash Bidadkosh
Jun 14, 2017·American Journal of Reproductive Immunology : AJRI·Sara HosseiniMohammad Salehi
Oct 14, 2017·Human Reproduction Update·Dulama Richani, Robert B Gilchrist

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