Improvement of Photorhabdus temperata strain K122 bioinsecticide production by batch and fed-batch fermentations optimization

Bioprocess and Biosystems Engineering
Wafa JallouliNabil Zouari

Abstract

Optimization of a fermentation process for bioinsecticides production by Photorhabdus temperata strain K122 was investigated into fully controlled 3-L fermenter using an optimized medium (OM). Development of large-scale inocula showed that the composition of the growth medium greatly influenced the physiological state of P. temperata cells. The effect of pH, agitation and dissolved oxygen concentration (DO) on the growth, culturability and oral toxicity of P. temperata cells were also investigated. Indeed, maintaining the pH at 7 and controlling DO concentration at 50 % saturation throughout the fermentation process, improved biomass production, CFU counts and oral toxicity by 41.1, 35 and 32.1 %, respectively, as compared to cultures carried out in 500 mL shake flasks. At such conditions, 8 g/L glucose fed-batch fermentation, enhanced cell lysis and variants small colony (Vsm) polymorphism appearance. To overcome such limitations, glucose concentration should be maintained at 4 g/L. In this case, P. temperata cells were produced at high cell density and culturability reaching 4.5 and 1.2 × 10(9) cells/mL, respectively. In addition, the stability of the primary form was maintained for a long period in the stationary growth phas...Continue Reading

References

Sep 1, 1969·Analytical Biochemistry·J B Lloyd, W J Whelan
Oct 13, 2000·Applied Microbiology and Biotechnology·T JeffkeL Berthe-Corti
Jun 11, 2011·Journal of Biomedicine & Biotechnology·Yonghong WangXing Zhang
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