Improvement of Potato virus Y (PVY) detection and quantitation using PVY(N)- and PVY(O)-specific real-time RT-PCR assays

Journal of Virological Methods
Valérie Balme-SinibaldiEmmanuel Jacquot

Abstract

A Potato virus Y (PVY) single nucleotide polymorphism (A/G(2213)), recently identified as a molecular determinant of the tobacco leaf necrosis symptom induced by PVY(N) isolates, has been used as a target to develop two PVY group-specific (PVY(N) and PVY(O)) fluorescent (TaqMan-based) real-time RT-PCR assays. These procedures allow detection, characterisation, and quantitation of a wide range of PVY isolates in samples containing 10(3)-10(8) viral transcripts. Moreover, the high specificity of these two new assays make the simultaneous detection and the reliable quantitation of PVY(N) and PVY(O) isolates in mixed solutions, regardless of the Y(N)/Y(O) ratio, feasible. The high sensitivity (threshold of 10(3) copies per reaction) and the PVY group specificity of these two new PVY detection tools clearly improve previously published PVY detection tests and offer new opportunities for PVY research programs.

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Citations

Mar 11, 2008·Journal of Virological Methods·P KogovsekM Ravnikar
Mar 11, 2008·Journal of Virological Methods·Fatima OsmanAdib Rowhani
Dec 22, 2006·Journal of Virological Methods·Jörg SchubertJoana Sztangret-Wiśniewska
Feb 9, 2013·Journal of Virological Methods·Kristina FlorschützGotthard Kunze
Feb 12, 2013·Journal of Virological Methods·Matevž RuparMaja Ravnikar
Apr 23, 2016·Journal of Virological Methods·Julie Villemot, Mathieu Rolland
Mar 17, 2017·Journal of Virological Methods·Fatima OsmanGeorgios Vidalakis
Oct 26, 2006·Journal of Agricultural and Food Chemistry·Giri Raj TripathiHyeonsook Cheong

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