Improvement of reproducibility and sensitivity by reducing matrix effect in micellar electrokinetic chromatography for determination of amino acids in turtle jelly

Electrophoresis
Lin-Qiu LiHon-Yeung Cheung

Abstract

Matrix effect (ME) is commonly seen in electrophoretic separation, but this phenomenon lacks any systematic study. Our work aimed to find out the relationship between separation efficiency and current, and then figure out an effective, simple, and economic solution to overcome the negative impact of ME. This present study showed that small amount of NaCl (≤0.005 mg/mL) in the sample had no impact on the separation but enhanced the sensitivity. However, when concentration of NaCl increased above 0.005 mg/mL, it alleviated the separation efficiency, sensitivity, and migration time. Besides, increasing NaCl concentration resulted in increasing turning point. The study of relationship of current and NaCl concentration indicated that when the TP of a sample is higher than 62.36 μA, desalination is necessary. Since the reported desalination methods are either expensive or complicated, we developed a simple and economic method by simply adding 12 times (volume) of chloroform/methanol (2:1, v/v) into the sample. When applied this method to turtle jelly, the number of theoretical plate (N) of 20 amino acids got up to threefold enhancement.

References

May 29, 2000·Electrophoresis·J P Schaeper, M J Sepaniak
Dec 25, 2002·Journal of Chromatography. a·E K Margareta Andersson, Ingela Hägglund
Jun 5, 2008·The Journal of Organic Chemistry·Qiliang Yuan, Dawei Ma
Oct 31, 2008·Talanta·Dayu LiuBingcheng Lin

❮ Previous
Next ❯

Citations

Jul 28, 2016·Electrophoresis·Andrea ŠlampováPetr Boček

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Related Papers

Journal of Separation Science
Koji Otsuka
Methods in Enzymology
N Matsubara, Shigeru Terabe
Molecular Biotechnology
Koji Otsuka, Shigeru Terabe
Methods in Molecular Biology
Koji Otsuka, Shigeru Terabe
© 2021 Meta ULC. All rights reserved