Improvement of the acridine orange-protein-surfactant system for protein estimation based on aromatic ring stacking effect of sodium dodecyl benzene sulphonate

Luminescence : the Journal of Biological and Chemical Luminescence
Fei WangZhen Jia

Abstract

The fluorescence of acridine orange (AO) is greatly quenched by the anionic surfactant sodium dodecyl benzene sulphonate (SDBS), but when protein is added into the AO-SDBS system, the fluorescence intensity of the latter is enhanced again. Based on this, a new fluorimetric method of determination of protein was developed. Under optimum conditions, the enhanced intensity of fluorescence is in proportion to the concentration of protein, such as bovine serum albumin (BSA), human serum albumin (HSA) and egg albumin (EA), over a wide range with detection limits at the 10(-9) g/mL level. This method has been satisfactorily used for the determination of protein in samples. We compared results using 280 nm and 490 nm excitation wavelengths and the mechanism of the assay.

References

Feb 1, 1995·Current Opinion in Biotechnology·R M Clegg
Jan 1, 1995·Methods in Enzymology·P R Selvin
Jan 23, 1998·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Q Z ZhuY B Zhao
May 16, 2000·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·W W QinY M Song
Nov 24, 2004·Luminescence : the Journal of Biological and Chemical Luminescence·Shufang LiuLijuan Mao
Feb 11, 2005·Organic & Biomolecular Chemistry·Keiji MizukiShigeori Takenaka

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Citations

Jul 5, 2007·Luminescence : the Journal of Biological and Chemical Luminescence·Zhanguang ChenYali Han
Jun 5, 2008·Luminescence : the Journal of Biological and Chemical Luminescence·Shuting SunQin Wei
Aug 13, 2013·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·J BhattacharjeeD Bhattacharjee

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