PMID: 11928927Apr 4, 2002Paper

Improvement of the culture conditions for in vitro production of cattle embryos in a portable CO2 incubator

Reproduction in Domestic Animals = Zuchthygiene
Y J DongT Suzuki

Abstract

The effects of different concentrations of growth hormone (GH) on in vitro maturation (IVM), fertilization (IVF) and culture (IVC) of bovine oocyte/embryos in CR1aa or CR2aa media using a simple CO2 incubator were investigated. The IVM/IVF/IVC of oocytes were carried out in the presence of 0, 50, 100 and 200 ng/ml GH in the medium. The proportion of metaphase II oocytes was significantly higher (p < 0.05) in 200 ng/ml compared with 0 ng/ml GH in CR1aa medium (59 versus 85%, respectively), but this effect was not observed under CR2aa. Higher concentrations of GH yielded lower rates of unfertilized ova and thus superior cleavage rates (36.5 +/- 0.2 and 63.5 +/- 2.0% versus 17.5 +/- 0.2 and 82.5 +/- 1.5% or 40.4 +/- 0.6 and 59.6 +/- 1.4% versus 16.6 +/- 1.2 and 83.4 +/- 6.2% for 0 and 200 ng/ml GH in portable or ordinary incubator, respectively) in CR1aa. This dose-dependent effect was also observed in the percentages of transferable embryos, although not statistically different (17.2 +/- 1.7 versus 27.3 +/- 1.8% and 16.6 +/- 3.1 versus 26.0 +/- 1.4%, for 0 versus 200 ng/ml GH in portable and ordinary incubator, respectively). In contrast to the CR1aa, different concentrations of GH in CR2aa medium did not increase either fertiliz...Continue Reading

References

Jan 1, 1979·Methods in Enzymology·R G Ham, W L McKeehan
Jan 1, 1991·Reproduction, Fertility, and Development·H G Gardner, P L Kaye
Mar 1, 1988·Endocrinology·M B Harvey, P L Kaye
Feb 1, 1987·Journal of Dairy Science·P D GluckmanS R Davis
Sep 1, 1993·Biology of Reproduction·C F RosenkransN L First
Sep 18, 1998·Molecular Reproduction and Development·L J HagemannH R Tervit
Mar 29, 2000·Theriogenology·B D Bavister

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