Improvement of the live vaccine strain Salmonella enterica serovar Typhi Ty21a for antigen delivery via the hemolysin secretion system of Escherichia coli

International Journal of Medical Microbiology : IJMM
Christian HotzIvaylo Gentschev

Abstract

The attenuated Salmonella enterica serovar Typhi strain Ty21a (Ty21a) is the only attenuated live oral vaccine against typhoid fever. Ty21a is also an attractive carrier for the delivery of heterologous antigens. We have used Ty21a for antigen delivery via the hemolysin (HlyA) secretion system of Escherichia coli, the prototype of the type I secretion system (T1SS). In this study, we identified by genetic complementation that the specific mutation of rpoS correlated with the hemolysin production of strain Ty21a. We furthermore showed that complementation with a plasmid encoding rfaH, which is described to be a downstream target of rpoS, led to increased expression and secretion of hemolysin. Finally, we demonstrated a significant enhancement of antibody responses against the heterologous HlyA antigen of Ty21a after immunization of mice with rfaH complemented S. typhi strain secreting HlyA compared with the same strain without rfaH plasmid.

Citations

Jan 16, 2014·Indian Journal of Microbiology·Bhagavathi Sundaram Sivamaruthi, Krishnaswamy Balamurugan
May 3, 2014·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Hidehiko SuzukiJun Kunisawa
Apr 8, 2010·Critical Reviews in Immunology·Roy CurtissShifeng Wang
Feb 5, 2014·FEBS Letters·Nicolás NavasaHonorina Martínez-Blanco
Nov 13, 2012·Microbial Pathogenesis·Shifeng WangRoy Curtiss

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