Improvement of the rapid immunoperoxidase staining method for intraoperative pathological diagnosis of pancreatic cancer using microwave irradiation.

Journal of Surgical Oncology
T IchiharaH Takagi

Abstract

The rapid immunostaining of gastrointestinal cancer-associated antigens, CA19-9, CEA, DUPAN2, and CA50, for intraoperative pathological diagnosis of pancreatic cancer can be completed in only 13 min by applying microwave irradiation, which was used to accelerate the incubation of the primary antibody. Only 3 s irradiation at 500 W for fresh-frozen sections produced specific antigen staining of greater intensity than that obtained with longer incubation, and tissue preservation was satisfactory with minimal non-specific background staining. This rapid immunoperoxidase staining method on the cryostat section of pancreatic biopsy specimens can provide important information for determining an appropriate operative method for pancreatic cancer patients.

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Citations

Jan 1, 1994·Micron : the International Research and Review Journal for Microscopy·M E Boon, L P Kok
Mar 1, 2003·Applied Immunohistochemistry & Molecular Morphology : AIMM·Robyn A BeachCynthia Cohen
Mar 17, 2006·Applied Immunohistochemistry & Molecular Morphology : AIMM·Elizabeth I JohnstonCynthia Cohen
Mar 1, 2005·Journal of Clinical Pathology·J HaapasaloJ Isola
Jun 1, 2006·World Journal of Surgery·Akimasa NakaoTsutomu Fujii
Mar 10, 2006·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·I-K LeeY H Ryu
Apr 17, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·U KämmererP Ruck
Nov 1, 1996·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·K SanoG Ma
Jul 22, 1998·Seminars in Surgical Oncology·A Nakao, H Takagi
Jul 22, 1998·Seminars in Surgical Oncology·A NakaoH Takagi

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