Improvement of the Rice "Easy-to-Shatter" Trait via CRISPR/Cas9-Mediated Mutagenesis of the qSH1 Gene

Frontiers in Plant Science
Xiabing ShengMeijuan Duan

Abstract

"Easy-to-shatter" trait is a major cause of rice crop yield losses, emphasizing the economic value of developing elite rice cultivars with reduced seed shattering capable of achieving higher yields. In the present study, we describe the development of new indica rice lines that exhibit lower rates of seed shattering following the targeted CRISPR/Cas9-mediated editing of the qSH1 gene. We were able to identify qSH1 mutant T0 transgenic plants, with transgene-free homozygous mutants being obtained via segregation in the T1 generation. We then utilized two T2 transgene-free homozygous lines in order to assess the degree of seed shattering and major agronomic traits of these mutant lines and of wild-type rice plants (HR1128-WT). This approach revealed that qsh1 homozygous mutant lines exhibited significantly reduced seed shattering relative to HR1128-WT without any significant changes in other analyzed agronomic traits. We then used these mutant lines to develop new promising hybrid rice lines with intermediate seed shattering. Overall our results reveal that combining targeted gene editing via CRISPR/Cas9 with heterosis utilization approach can allow for the efficient development of novel promising hybrid rice cultivars that exhib...Continue Reading

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Citations

Feb 10, 2021·Plant Cell Reports·Xin HuangChangfu Zhu
Jul 16, 2021·Transgenic Research·Jerlie Mhay MatresInez H Slamet-Loedin
Jul 22, 2021·Journal of Agricultural and Food Chemistry·Shakeel AhmadShaoqing Tang
Aug 23, 2021·Molecular Biotechnology·Dipinte GuptaRajiv Ranjan
Aug 29, 2021·Plants·Yang ZhaoJianping Wang

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Methods Mentioned

BETA
transgenic
PCR
electrophoresis
light microscopy
targeted mutations

Software Mentioned

DNAMAN5
MAGE4

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