PMID: 8985384Jan 1, 1997Paper

Improvement of the specific infectivity of the rubella virus (RUB) infectious clone: determinants of cytopathogenicity induced by RUB map to the nonstructural proteins

Journal of Virology
K V PugachevTeryl K Frey

Abstract

A plasmid, Robo102, which contains a cDNA copy of the rubella virus (RUB) genomic RNA from which infectious transcripts can be synthesized in vitro, was recently developed (C. Y. Wang, G. Dominguez, and T. K. Frey, J. Virol. 68:3550-3557, 1994). To increase the specific infectivity of Robo102 transcripts (approximately 5 plaques/10 microg of transcripts), a modified reverse transcription-PCR method was used to amplify nearly 90% of the RUB genome in three fragments, which were then used to replace the corresponding fragments in Robo102. Replacement of a fragment covering nucleotides (nt) 5352 to 9759 of the RUB genome yielded a construct, Robo202, which produced highly infectious transcripts (10(4) plaques/microg), indicating the presence of an unrecognized deleterious mutation (or mutations) in this region of the Robo102 cDNA. Robo102 was based on the w-Therien strain of RUB, which forms opaque plaques in Vero cells, while the PCR replacement fragments were generated from a variant, f-Therien, which produces clear plaques in Vero cells. Although Robo202 contains over 4,000 nt from f-Therien, Robo202 virus produces opaque plaques. However, when the other two PCR fragments amplified from f-Therien (nt 1 to 1723 and nt 2800 to 53...Continue Reading

References

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Citations

Mar 9, 2002·Journal of Virology·Wen-Pin Tzeng, Teryl K Frey
Jul 20, 2004·Birth Defects Research. Part A, Clinical and Molecular Teratology·C D AtreyaS Kulkarni
Jun 1, 2014·The Journal of General Virology·Rodion V GorchakovFarooq Nasar
Mar 30, 2004·Journal of Virology·Min-Hsin Chen, Joseph P Icenogle
Jan 13, 2012·The Journal of General Virology·Suganthi SuppiahTeryl K Frey
Aug 31, 1999·The Journal of General Virology·E ten DamM D Ryan
Sep 21, 2013·Journal of Virology·Bunpote SiridechadilokJuthathip Mongkolsapaya
Jan 21, 2010·The Journal of Biological Chemistry·Yubin ZhouJenny J Yang
Aug 14, 2013·PloS One·Ludmila PerelyginaJoseph Icenogle
Mar 12, 1999·Journal of Virology·M H Chen, T K Frey
Oct 30, 1998·Virology·K V Pugachev, T K Frey
Oct 12, 2000·Clinical Microbiology Reviews·J Y Lee, D S Bowden
May 21, 2011·Virology Journal·Suganthi SuppiahTeryl K Frey

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