Improvement of the stability of alcohol dehydrogenase by covalent immobilization on glyoxyl-agarose

Journal of Biotechnology
Juan Manuel BolivarCesar Mateo

Abstract

Immobilization of alcohol dehydrogenase (ADH) from Horse Liver inside porous supports promotes a dramatic stabilization of the enzyme against inactivation by air bubbles in stirred tank reactors. Moreover, immobilization of ADH on glyoxyl-agarose promotes additional stabilization against any distorting agent (pH, temperature, organic solvents, etc.). Stabilization is higher when using highly activated supports, they are able to immobilize both subunits of the enzyme. The best glyoxyl derivatives are much more stable than conventional ADH derivatives (e.g., immobilized on BrCN activated agarose). For example, glyoxyl immobilized ADH preserved full activity after incubation at pH 5.0 for 20h at room temperature and conventional derivatives (as well as the soluble enzyme) preserved less than 50% of activity after incubation under the same conditions. Moreover, glyoxyl derivatives are more than 10 times more stable than BrCN derivatives when incubated in 50% acetone at pH 7.0. Multipoint covalent immobilization, in addition to multisubunit immobilization, seems to play an important stabilizing role against distorting agents. In spite of these interesting stabilization factors, immobilization hardly promotes losses of catalytic acti...Continue Reading

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Citations

Jun 12, 2008·Applied Microbiology and Biotechnology·Juan M BolivarRoberto Fernandez-Lafuente
Jan 20, 2011·Applied Microbiology and Biotechnology·Ye-Wang ZhangJung-Kul Lee
Feb 19, 2013·Biotechnology Letters·Youhui LiQipeng Yuan
May 16, 2009·Applied Biochemistry and Biotechnology·Abolfazl BarzegarNader Sheibani
Jan 8, 2010·Applied Biochemistry and Biotechnology·Melda AltikatogluHuriye Kuzu
Mar 25, 2014·Applied Microbiology and Biotechnology·Swati B JadhavShrikant A Survase
Jan 25, 2012·Colloids and Surfaces. B, Biointerfaces·Joey N Talbert, Julie M Goddard
Jan 23, 2014·Biotechnology Progress·J GrimaldiG Belfort
Feb 27, 2015·Journal of Biomaterials Science. Polymer Edition·Deniz Aktaş UygunAdil Denizli
Dec 24, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Joseph GrimaldiGeorges Belfort
Jul 7, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Gökay Vardarİbrahim Işıldak
Jan 12, 2013·International Journal of Molecular Sciences·Raushan Kumar SinghJung-Kul Lee
Dec 2, 2010·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Melda Altikatoglu, Mithat Celebi
May 22, 2021·Applied Biochemistry and Biotechnology·Sabrina Ait BrahamRoberto Fernandez-Lafuente
Jun 21, 2021·Biotechnology Advances·David RibeaucourtJean-Guy Berrin
Jul 6, 2021·Green Chemistry : an International Journal and Green Chemistry Resource : GC·Maria Romero-Fernandez, Francesca Paradisi
Aug 30, 2021·Biotechnology Advances·Rafael C RodriguesRoberto Fernandez-Lafuente
Dec 8, 2009·Carbohydrate Research·Abolfazl BarzegarSaman Hosseini Ashtiani

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