Improvement of the total turnover number and space-time yield for chloroperoxidase catalyzed oxidation

Biotechnology and Bioengineering
K SeelbachU Kragl


Chloroperoxidase from Caldariomyces fumago was applied for the oxidation of indole to oxindole using hydrogen peroxide as the oxidant in aqueous t-butyl alcohol medium. Different ways of adding the oxidant, various reactor types, and the use of a hydrogen peroxide-stat were compared, resulting in a 20-fold increase of the total turnover number (ttn) and space-time yield (sty). The highest ttn of >860,000 was obtained in a fed-batch reactor, whereas the highest sty of 120 g/(L . d) was reached in a continuously operated enzyme membrane reactor. The results were compared to other enzyme systems already established for the synthesis of amino acids and carbohydrates. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55: 283-288, 1997.


Nov 1, 1979·The Biochemical Journal·M D Corbett, B R Chipko
Nov 30, 1992·Annals of the New York Academy of Sciences·Andreas S BommariusC Wandrey
Apr 29, 1986·Biochemical and Biophysical Research Communications·J GeigertS L Neidleman
Sep 1, 1984·Archives of Biochemistry and Biophysics·G L Kedderis, P F Hollenberg
Aug 12, 1983·Biochemical and Biophysical Research Communications·J GeigertJ Wadsworth
Jun 1, 1995·Archives of Biochemistry and Biophysics·V P MillerP R Ortiz de Montellano
Oct 20, 1996·Biotechnology and Bioengineering·U KraglC Wandrey


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Chloride Peroxidase
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