Improvement of the total turnover number and space-time yield for chloroperoxidase catalyzed oxidation

Biotechnology and Bioengineering
K SeelbachU Kragl

Abstract

Chloroperoxidase from Caldariomyces fumago was applied for the oxidation of indole to oxindole using hydrogen peroxide as the oxidant in aqueous t-butyl alcohol medium. Different ways of adding the oxidant, various reactor types, and the use of a hydrogen peroxide-stat were compared, resulting in a 20-fold increase of the total turnover number (ttn) and space-time yield (sty). The highest ttn of >860,000 was obtained in a fed-batch reactor, whereas the highest sty of 120 g/(L . d) was reached in a continuously operated enzyme membrane reactor. The results were compared to other enzyme systems already established for the synthesis of amino acids and carbohydrates. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55: 283-288, 1997.

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Citations

Jun 12, 2013·Applied Microbiology and Biotechnology·Milja PešićGregorio Alvaro
Jan 24, 2015·Biotechnology and Bioengineering·Andreas BosshartMatthias Bechtold
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Nov 9, 2005·Biotechnology and Bioengineering·Jin-Byung Park, Douglas S Clark
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Jan 12, 2019·Applied Microbiology and Biotechnology·Svenja BloessGabriele Fischer von Mollard

Related Concepts

Chloride Peroxidase
Hydrogen Peroxide
Laboratory
Oxidation
Oxides
2-oxindole
Carbohydrate Food
Leptoxyphium fumago
TTN

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