PMID: 58960Jan 1, 1976

Improvements and simplifications of the ozonolysis-Schiff staining method for plasma lipoproteins on cellulose acetate

Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie
A Uldall


Modifications of the ozonolysis and Schiff staining of plasma lipoproteins reduce 11 pre-spectrometric steps to 3. Picrate denaturation of the lipoproteins and absorption spectrometry directly on the wet opaque membrane increase the sensitivity; the volume of sub-specimen needed per length of application site is reduced from 0.7 to 0.1 mul/mm. Reasonably good proportionality between the total lipoprotein concentration and the spectrometric findings are obtained. Two specimens were obtained at an interval of 8-13 days from each of 10 patients; for the arbitrary substance fraction of alpha-, prebeta-, and beta-lipoproteins in plasma the between-series intra-individual standard deviations were 0.021, 0.023 and 0.032 respectively.


Dec 1, 1968·Clinica Chimica Acta; International Journal of Clinical Chemistry·T Postma, J A Stroes

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Electrophoresis, Cellulose Acetate
Alpha-1 Lipoprotein
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Staining and Labeling

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