PMID: 6168035May 1, 1981Paper

Improvements in histological techniques for epoxy-resin embedded bone specimens

Stain Technology
R H WattsG R Howells

Abstract

A procedure is presented in which some of the processing difficulties with fixation, embedding and cutting whole mouse bones and large bone pieces from other species are considered. The bone specimens are fixed in acetone or by a Karnovsky-formol-saline process which preserves intact endosteal surface-to-cortex layers. After fixation the bones are embedded in a hard mixture of epoxy resin to provide blocks with face sizes up to 3.5 x 3.0 cm. Mineralized sections are cut to 4 micrometer; demineralized at 3 micrometer. Sections are fastened to gelatin-subbed slides with pressure plates which produce flat, secure sections. After removal of the plastic, an unmodified Mayer's hematoxylin and a polychromatic eosin staining method is applied to demineralized sections, and a slightly modified method to mineralized sections.

References

Jan 1, 1969·Journal of Ultrastructure Research·A R Spurr

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Citations

Jan 1, 1991·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·L N JensenK Gotfredsen
Mar 1, 1995·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·V GrillP Narducci
May 17, 2011·Acta histochemica·Jin Ik Lim, Hun-Kuk Park
May 21, 2009·Microscopy Research and Technique·Jin Ik Lim, Yong-Keun Lee
Jan 12, 2010·Microscopy Research and Technique·Jin Ik LimYong-Keun Lee
May 1, 1984·Journal of Biomedical Materials Research·T A Gruen, A Sarmiento

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