Improvements in ion signal reproducibility obtained using a homogeneous laser beam for on-line laser desorption/ionization of single particles

Rapid Communications in Mass Spectrometry : RCM
Ryan J Wenzel, Kimberly A Prather

Abstract

A major factor limiting on-line single particle mass spectrometry techniques from becoming more quantitative is the large shot-to-shot variability in ion intensities observed in the laser desorption/ionization (LDI) mass spectra.1,2 In previous work, lab-generated particles showed fluctuations of up to 152% in the absolute ion intensities in averaged spectra of 200-300 'identical' particles.2 Most of these fluctuations were attributed to inhomogeneities in the laser beam profile, leading to significant differences in the power each particle encountered depending on the position in the LDI laser beam where it underwent analysis. The goal of the work presented herein is to determine whether a fiber optic actually reduces the observed variability in single particle LDI mass spectral data. Initial results are presented for individual single component organic particles composed of 2,4-dihydroxybenzoic acid (2,4-DHB) analyzed using a low-power flat-top laser beam profile created by sending an ultraviolet (266 nm) DI laser through a fiber optic. Relative standard deviations of the total ion intensities for peaks in individual spectra are reduced to 31%. Single particle spectra, compared with and without laser homogenization at the sam...Continue Reading

Citations

Feb 15, 2013·Analytical and Bioanalytical Chemistry·Joseph P Klems, Murray V Johnston
May 28, 2013·Journal of the American Society for Mass Spectrometry·Rory T StevenJosephine Bunch
Dec 25, 2007·Journal of the American Society for Mass Spectrometry·Erica L McJimpseyPaul T Steele
Jul 19, 2008·Annual Review of Analytical Chemistry·Kimberly A PratherVicki H Grassian
Oct 18, 2006·Mass Spectrometry Reviews·Daniel M Murphy
Jun 26, 2007·Journal of Mass Spectrometry : JMS·Klaus-Peter Hinz, Bernhard Spengler
Aug 23, 2016·Environmental Science & Technology·Jessica L AxsonAndrew P Ault
Sep 4, 2009·Physical Chemistry Chemical Physics : PCCP·Ryan C SullivanKimberly A Prather
Nov 28, 2020·Environmental Pollution·Jovanna ArndtJohn C Wenger
Feb 27, 2021·Rapid Communications in Mass Spectrometry : RCM·Chaowu LiuZhen Zhou
Jan 30, 2009·Analytical Chemistry·Kerri A PrattKimberly A Prather
Jul 29, 2005·Environmental Science & Technology·David A SodemanKimberly A Prather

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