Improvements in protein production in mammalian cells from targeted metabolic engineering

Current Opinion in Systems Biology
Anne Richelle, Nathan E Lewis

Abstract

Bioprocess optimization has yielded powerful clones for biotherapeutic production. However, new genomic technologies allow more targeted approaches to cell line development. Here we review efforts to enhance protein production in mammalian cells through metabolic engineering. Most efforts aimed to reduce toxic byproducts accumulation to enhance protein productivity. However, recent work highlights the possibility of regulating other desirable traits (e.g., apoptosis and glycosylation) by targeting central metabolism since these processes are interconnected. Therefore, as we further detail the pathways underlying cell growth and protein production and deploy diverse algorithms for their analysis, opportunities will arise to move beyond simple cell line designs and facilitate cell engineering strategies with complex combinations of genes that together underlie a phenotype of interest.

Citations

Apr 29, 2018·Biotechnology and Bioengineering·Oliver RuppKelvin H Lee
Aug 17, 2019·Applied Microbiology and Biotechnology·Iman Shahidi Pour SaviziSeyed Abbas Shojaosadati
Jan 11, 2020·Bulletin of Mathematical Biology·Luis Sordo VieiraDavid Murrugarra
Jun 22, 2019·Scientific Reports·Austin W T ChiangNathan E Lewis
May 9, 2019·Biotechnology Letters·N JiménezZ P Gerdtzen
Feb 11, 2020·Frontiers in Immunology·Sharon Yehuda, Vered Padler-Karavani
Mar 27, 2020·Bioprocess and Biosystems Engineering·Hamideh FouladihaBehrouz Vaziri
Dec 10, 2017·Current Opinion in Biotechnology·Chih-Chung KuoNathan E Lewis
Oct 17, 2020·Omics : a Journal of Integrative Biology·Nguyen Hoang AnhNguyen Phuoc Long

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