Improving cellularity and quality of liquid-based cytology slides processed from pancreatobiliary tract brushings

Diagnostic Cytopathology
Michael B CampionMichael R Henry

Abstract

Cytology has been reported to have suboptimal sensitivity for detecting pancreatobiliary tract cancer in biliary tract specimens partly as a result of low specimen cellularity and obscuring noncellular components. The goal of this study was to determine if the use of a glacial acetic acid wash prior to processing would increase the cellularity and improve the quality of ThinPrep slides when compared to standard non-gyn ThinPrep processing. Fifty consecutive pancreatobiliary tract specimens containing 20 ml of sample/PreservCyt were divided equally for standard non-gyn ThinPrep (STP) and glacial acetic acid ThinPrep processing (GATP). A manual drop preparation was also performed on residual STP specimen to determine the number of cells left in the vial during STP processing. Twenty-six (52%) specimens had more epithelial cell groupings with the GATP methodology while 19 (38%) had equivalent cellularity with both methods. The STP method produced more epithelial cell groupings in 5 (10%) of the specimens. Of the 26 specimens that had less cells with the STP method, 14 (54%) had > or = 50 cell groupings on the manual drop slide processed from the residual STP specimen suggesting that many cells remain in the vial after STP processi...Continue Reading

References

Mar 13, 2002·American Journal of Clinical Pathology·Joel S BentzC Jay Marshall
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Apr 2, 2004·Cytopathology : Official Journal of the British Society for Clinical Cytology·C O WightS D Ryder
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Feb 21, 2008·World Journal of Gastroenterology : WJG·Andreas WeberChristian Prinz

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Citations

Jul 31, 2014·Cytopathology : Official Journal of the British Society for Clinical Cytology·E G Barr FritcherA C Clayton
May 4, 2018·Cytopathology : Official Journal of the British Society for Clinical Cytology·Y NaitoJ Akiba
Sep 14, 2017·The Korean Journal of Internal Medicine·Myeong Ho YeonHo Chang Lee
May 2, 2019·Diagnostic Cytopathology·Andrew A Renshaw, Edwin W Gould

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