Improving derivatization efficiency of BMAA utilizing AccQ-Tag in a complex cyanobacterial matrix

Amino Acids
Johan ErikssonBirgitta Bergman

Abstract

Two different assays have been developed and used in order to investigate the optimal conditions for derivatization and detection of acid beta-N-methyl-amino-L-alanine (BMAA) in a cyanobacterial sample. BMAA was extracted from cyanobacterial cultures both from the cytosolic ("free") fraction and in the precipitated ("protein") fraction using a newly developed extraction scheme and the sample matrix was standardized according to protein concentration to ensure the highest possible derivative yield. A rapid and sensitive HPLC method for fluorescence detection of the non-protein amino acid BMAA in cyanobacteria, utilizing the Waters AccQ-Tag chemistry and Chromolith Performance RP-18e columns was developed. Using this new method and utilizing a different buffer system and column than that recommended by Waters, we decreased the time between injections by 75%. The limit of quantification was determined to be 12 nmol and limit of detection as 120 fmol. The linear range was in the range of 8.5 nmol-84 pmol. Accuracy and precision were well within FDA guidelines for bioanalysis.

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Citations

May 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sara JonassonBirgitta Bergman
Apr 25, 2012·Analytical and Bioanalytical Chemistry·Liying JiangLeopold L Ilag
Aug 25, 2011·Amino Acids·Rosa C Cervantes CiancaVitor M Vasconcelos
Dec 16, 2009·Amyotrophic Lateral Sclerosis : Official Publication of the World Federation of Neurology Research Group on Motor Neuron Diseases·Walter G Bradley, Deborah C Mash
Dec 16, 2009·Amyotrophic Lateral Sclerosis : Official Publication of the World Federation of Neurology Research Group on Motor Neuron Diseases·Ran Cheng, Sandra Anne Banack
Dec 16, 2009·Amyotrophic Lateral Sclerosis : Official Publication of the World Federation of Neurology Research Group on Motor Neuron Diseases·Paul Alan Cox
Aug 26, 2015·Environmental Science and Pollution Research International·Sandra LageSara Rydberg
Jan 17, 2012·Toxicon : Official Journal of the International Society on Toxinology·R C Cervantes CiancaV M Vasconcelos
Jun 22, 2010·Toxicon : Official Journal of the International Society on Toxinology·S A BanackP A Cox
Oct 22, 2013·Toxicon : Official Journal of the International Society on Toxinology·Maitham Ahmed Al-SammakDavid Cassada
Feb 19, 2011·Toxicon : Official Journal of the International Society on Toxinology·S A BanackP A Cox
Aug 30, 2011·Toxicon : Official Journal of the International Society on Toxinology·Mafalda S BaptistaVitor M Vasconcelos
Dec 22, 2009·The Analyst·Zdenek SpácilBirgitta Bergman
Sep 23, 2016·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Cristina PorojanAmbrose Furey
Nov 19, 2018·Journal of Hazardous Materials·Korina ManolidiAnastasia Hiskia

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