Improving draft assemblies by iterative mapping and assembly of short reads to eliminate gaps

Genome Biology
Isheng J TsaiMatthew Berriman

Abstract

Advances in sequencing technology allow genomes to be sequenced at vastly decreased costs. However, the assembled data frequently are highly fragmented with many gaps. We present a practical approach that uses Illumina sequences to improve draft genome assemblies by aligning sequences against contig ends and performing local assemblies to produce gap-spanning contigs. The continuity of a draft genome can thus be substantially improved, often without the need to generate new data.

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Related Concepts

Sequence Determinations, DNA
Contig Mapping
Genomics
Genome
Local
Molecular Assembly/Self Assembly
Nucleic Acid Sequencing
probe gene fragment

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