May 30, 2020

Improving Gene Therapy Efficacy Using Impella

The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation
S TharakanK Ishikawa

Abstract

Gene therapy is a promising option for heart failure patients, but there remains a lack of effective delivery mechanisms. The Impella device is a heart pump that can increase coronary flow and pressure, important factors that determines efficacy of gene transduction when using intracoronary delivery. Because it also lends hemodynamic support, the Impella can be coupled with balloon coronary occlusion to increase cardiac vector dwell time. We hypothesized that use of the Impella offers safe and efficient delivery of genes using intracoronary administration of adeno-associated viral (AAV)-6. AAV6 encoding luciferase gene (5.0 × 10E13) was injected into the coronary artery one week after MI induction in Yorkshire pigs. A total of twelve pigs were allocated to one of the following groups: slow antegrade delivery (n=3), slow antegrade delivery with Impella (n=3), delivery during coronary artery occlusion with Impella hemodynamic support (n=3), delivery during coronary artery and sinus occlusions with Impella hemodynamic support (CA + CS, n=3), Four weeks later, tissues were harvested from various heart regions and other organs to assess luciferase expression and viral uptake. Impella support offered safe vector delivery during 1-2 m...Continue Reading

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Mentioned in this Paper

Genes
Genome
Signal Transduction
Heart Tissue
Myocardial Infarction
Liver
Insulin Pump
Organ
Protein Expression
Heart Failure

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