Improving lupeol production in yeast by recruiting pathway genes from different organisms

Scientific Reports
Weibo QiaoYansheng Zhang

Abstract

Lupeol is a pentacyclic triterpene that shows a variety of pharmacological properties. Compared to engineering the production of sesquiterpenes and diterpenes, it is much more challenging to engineer the biosynthesis of triterpenes in microbial platforms. This study showed our efforts on engineering the triterpene pathway in Escherichia coli and Saccharomyces cerevisiae cells by recruiting the codon-optimized three lupeol pathway genes from different organisms. By comparing their activities with their respective counterparts, the squalene synthase from Thermosynechococcus elongates (tSQS), the squalene epoxidase from Rattus norvegicus (rSE) and the lupeol synthase from Olea europaea (OeLUP) were introduced into E. coli BL21(DE3), a break-through from zero was observed for lupeol biosynthesis in a prokaryotic host. We also assessed the lupeol pathway under two different yeast backgrounds-WAT11 and EPY300, and have found that the engineered strains based on EPY300, named ECHHOe, processed the best lupeol-producing ability with the maximum lupeol titer being 200.1 mg l-1 at 30 °C in a 72 h-flask culture, which so far was the highest amount of lupeol obtained by a microbial system and provides a basis for further industrial applica...Continue Reading

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Citations

Jul 22, 2020·Biotechnology and Bioengineering·Yunhe MengChonglong Wang
Aug 5, 2020·Environmental Science and Pollution Research International·Maria da Conceição MarinhoSara Cristina Antunes
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jan Bachořík, Milan Urban
Jul 9, 2021·Journal of Biotechnology·Jennifer Hage-HülsmannThomas Drepper
Aug 28, 2021·ACS Synthetic Biology·Weibo QiaoYansheng Zhang

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Methods Mentioned

BETA
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