Improving mass spectrometric sequencing of arginine-containing peptides by derivatization with acetylacetone

Journal of Mass Spectrometry : JMS
S DiklerD H Russell

Abstract

Modification of arginine residues in bradykinin, [1-5]-bradykinin, splenopentin and two synthetic pentapeptides with acetylacetone (pentane-2,4-dione) significantly increases the relative abundance of sequence-specific fragment ions produced by matrix-assisted laser desorption/ionization (MALDI). The fragmentation efficiency as measured by post-source decay in a reflectron time-of-flight mass spectrometer increases by a factor of 2-3.5. Peptide bonds adjacent to modified residues are more susceptible to cleavage than in the non-derivatized peptide ions. The increased lability of these bonds gives rise to more complete sequence information. In addition, the relative abundances of sequence-specific fragment ions are enhanced. This strategy makes it possible to obtain valuable structural information from arginine-containing peptides that otherwise do not fragment well.

Citations

Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·T KeoughM P Lacey
Apr 10, 2007·Journal of the American Society for Mass Spectrometry·Yi-Min SheJohn B Westmore
Nov 3, 2009·Journal of Proteome Research·Junmei ZhangYingming Zhao
Aug 26, 2003·Journal of Mass Spectrometry : JMS·Alexander Leitner, Wolfgang Lindner
Oct 7, 2003·Mass Spectrometry Reviews·Simone Cristoni, Luigi Rossi Bernardi
Mar 17, 2006·Journal of Mass Spectrometry : JMS·Alexandra FoettingerWolfgang Lindner
Jun 4, 2008·Rapid Communications in Mass Spectrometry : RCM·Hiroki KuyamaSusumu Tsunasawa
Apr 14, 2011·Rapid Communications in Mass Spectrometry : RCM·Masahiro MiyashitaHisashi Miyagawa
Jul 9, 2013·Journal of Mass Spectrometry : JMS·William M McGee, Scott A McLuckey
Oct 12, 2010·The Analyst·Chihiro NakajimaSusumu Tsunasawa
Apr 29, 2000·Electrophoresis·K Gevaert, J Vandekerckhove
Jul 25, 2013·Molecular & Cellular Proteomics : MCP·Alexander S HebertJoshua J Coon
Dec 24, 2018·Chembiochem : a European Journal of Chemical Biology·Heejin RohSeokhee Kim
Mar 31, 2000·Trends in Biotechnology·L LiJ V Sweedler
Jun 29, 1999·Journal of the American Society for Mass Spectrometry·A G BakerM V Novotny
Jan 5, 2002·Journal of the American Society for Mass Spectrometry·B B Willard, M Kinter
May 18, 2004·Journal of the American Society for Mass Spectrometry·Kevin A SchugKarel Lemr
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Junmei ZhangHaydn L Ball
Jun 1, 2002·Analytical Chemistry·Volker SchnaibleDaniel Hoffmann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.