Improving nuclear envelope dynamics by EBV BFRF1 facilitates intranuclear component clearance through autophagy

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Guan-Ting LiuChung-Pei Lee

Abstract

Although a vesicular nucleocytoplasmic transport system is believed to exist in eukaryotic cells, the features of this pathway are mostly unknown. Here, we report that the BFRF1 protein of the Epstein-Barr virus improves vesicular transport of nuclear envelope (NE) to facilitate the translocation and clearance of nuclear components. BFRF1 expression induces vesicles that selectively transport nuclear components to the cytoplasm. With the use of aggregation-prone proteins as tools, we found that aggregated nuclear proteins are dispersed when these BFRF1-induced vesicles are formed. BFRF1-containing vesicles engulf the NE-associated aggregates, exit through from the NE, and putatively fuse with autophagic vacuoles. Chemical treatment and genetic ablation of autophagy-related factors indicate that autophagosome formation and autophagy-linked FYVE protein-mediated autophagic proteolysis are involved in this selective clearance of nuclear proteins. Remarkably, vesicular transport, elicited by BFRF1, also attenuated nuclear aggregates accumulated in neuroblastoma cells. Accordingly, induction of NE-derived vesicles by BFRF1 facilitates nuclear protein translocation and clearance, suggesting that autophagy-coupled transport of nucleus...Continue Reading

References

Jan 1, 1983·The Journal of Cell Biology·R M SteinmanZ A Cohn
Apr 30, 2002·Human Molecular Genetics·Brinda RavikumarDavid C Rubinsztein
Oct 24, 2002·Human Molecular Genetics·Wen YangRonald Wetzel
Jan 17, 2003·Molecular Biology of the Cell·Paul RobertsDavid S Goldfarb
Mar 3, 2004·Nature Reviews. Molecular Cell Biology·Lan Xu, Joan Massagué
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Yosef GruenbaumKatherine L Wilson
Apr 28, 2005·Current Biology : CB·Daniel J Klionsky
Aug 2, 2005·Molecular Biology of the Cell·Lianwu FuElizabeth Sztul
Sep 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Atsushi IwataRon R Kopito
Nov 17, 2005·Nature Reviews. Neuroscience·Elena CattaneoMarzia Tartari
Jul 28, 2006·Methods : a Companion to Methods in Enzymology·Michael J Matunis
Sep 16, 2006·Molecular Cell·Christian BehrendsF Ulrich Hartl
Jan 24, 2007·Lancet·Francis O Walker
Oct 24, 2007·Analytical Biochemistry·Edward Chang, Jeff Kuret
Jun 6, 2008·The Journal of Biological Chemistry·Yoshinobu IchimuraMasaaki Komatsu
Feb 10, 2009·Methods in Enzymology·Geir BjørkøyTerje Johansen
Jun 25, 2009·Autophagy·Young-Eun ParkIchizo Nishino
Feb 18, 2012·Nature Reviews. Cancer·Kin-Hoe ChowKatharine S Ullman
Jun 2, 2012·Cell Death and Differentiation·P IsaksonA Simonsen
Jul 7, 2012·PloS One·Dalibor MijaljicaRodney J Devenish
Aug 4, 2012·Trends in Cell Biology·April Rose, Christian Schlieker
Aug 8, 2013·Nature Medicine·Ralph A Nixon
Sep 28, 2013·Essays in Biochemistry·Moran Rawet Slobodkin, Zvulun Elazar
Nov 29, 2014·The FEBS Journal·Bastien CautainWolfgang Link
Jun 26, 2015·Journal of Virology·Janna M Bigalke, Ekaterina E Heldwein

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Citations

Jul 22, 2018·Biochemical Society Transactions·David J Thaller, C Patrick Lusk
Sep 23, 2018·Protein & Cell·Ying LvHongyu Deng
May 1, 2021·Viruses·Chung-Pei Lee, Mei-Ru Chen
Jun 3, 2021·Viruses·Mai Tram Vo, Young Bong Choi

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