PMID: 8956336Nov 1, 1996Paper

Improving protein delivery from microparticles using blends of poly(DL lactide co-glycolide) and poly(ethylene oxide)-poly(propylene oxide) copolymers

Pharmaceutical Research
M K YehA G Coombes

Abstract

Microparticles containing ovalbumin as a model for protein drugs were formulated from blends of poly(DL lactide-co-glycolide) and poly(ethylene oxide)-poly(propylene oxide) copolymers (Pluronic). The objectives were to achieve uniform release characteristics and improved protein delivery capacity. The water- in oil -in oil emulsion/solvent extraction technique was used for microparticle production. A protein loading level of over 40% (w/w) was attained in microparticles having a mean diameter of approximately 5 microns. Linear protein release profiles over 25 days in vitro were exhibited by certain blend formulations incorporating hydrophilic Pluronic F127. The release profile tended to plateau after 10 days when the more hydrophobic Pluronic L121 copolymer was used to prepare microparticles. A delivery capacity of 3 micrograms OVA/mg particles/ day was achieved by formulation of microparticles using a 1:2 blend of PLG:Pluronic F127. The w/o/o formulation approach in combination with PLG:Pluronic blends shows potential for improving the delivery of therapeutic proteins and peptides from microparticulate systems. Novel vaccine formulations are also feasible by incorporation of Pluronic L121 in the microparticles as a co-adjuvant.

Citations

Apr 6, 2006·Journal of Biomedical Materials Research. Part a·Yuancai Dong, Si-Shen Feng
Jan 7, 1999·Drug Development and Industrial Pharmacy·R JainC T Rhodes
Apr 19, 2007·Journal of Microencapsulation·Ming-Kung YehZhi-Yang Chang
Aug 29, 2018·Chemistry, an Asian Journal·Guixian ZhuLianqing Zhu
Jun 2, 2015·PloS One·Manoj S NairMichael K Chan
Mar 21, 2002·The Journal of Pharmacy and Pharmacology·Caroline PérezKai Griebenow
Oct 6, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·Z MiP D Robbins
Jan 26, 2006·International Journal of Pharmaceutics·A T Raiche, D A Puleo
Dec 29, 2007·International Journal of Pharmaceutics·A GiteauJ P Benoit

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