Improving qPCR telomere length assays: Controlling for well position effects increases statistical power

American Journal of Human Biology : the Official Journal of the Human Biology Council
Dan T A EisenbergM Geoffrey Hayes

Abstract

Telomere length (TL) is commonly measured using quantitative PCR (qPCR). Although, easier than the southern blot of terminal restriction fragments (TRF) TL measurement method, one drawback of qPCR is that it introduces greater measurement error and thus reduces the statistical power of analyses. To address a potential source of measurement error, we consider the effect of well position on qPCR TL measurements. qPCR TL data from 3,638 people run on a Bio-Rad iCycler iQ are reanalyzed here. To evaluate measurement validity, correspondence with TRF, age, and between mother and offspring are examined. First, we present evidence for systematic variation in qPCR TL measurements in relation to thermocycler well position. Controlling for these well-position effects consistently improves measurement validity and yields estimated improvements in statistical power equivalent to increasing sample sizes by 16%. We additionally evaluated the linearity of the relationships between telomere and single copy gene control amplicons and between qPCR and TRF measures. We find that, unlike some previous reports, our data exhibit linear relationships. We introduce the standard error in percent, a superior method for quantifying measurement error as c...Continue Reading

References

Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J MeyneR K Moyzis
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R K MoyzisJ R Wu
May 10, 2002·Nucleic Acids Research·Richard M Cawthon
Mar 6, 2003·Neuroscience Letters·Christian RamakersAntoon F M Moorman
Feb 25, 2009·Nucleic Acids Research·J M RuijterA F M Moorman
Jan 12, 2010·International Journal of Epidemiology·Silvia EhrlenbachAnita Brandstätter
May 29, 2010·International Journal of Epidemiology·Linda S AdairMichelle J Hindin
Jul 8, 2010·JAMA : the Journal of the American Medical Association·Peter WilleitStefan Kiechl
Feb 15, 2011·American Journal of Human Biology : the Official Journal of the Human Biology Council·Dan T A Eisenberg
Jun 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Dan T A EisenbergChristopher W Kuzawa
Sep 11, 2013·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Julie M CunninghamLisa A Boardman
Dec 25, 2013·Experimental Gerontology·Michael GardnerUNKNOWN Halcyon study team
May 7, 2014·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Lisa A BoardmanKristin Litzelman
May 7, 2014·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Jonathan N HofmannMark P Purdue
Jun 18, 2014·Pediatrics·Stacy S DruryKatherine P Theall
Jul 10, 2014·BMJ : British Medical Journal·Philip C HaycockPeter Willeit
Sep 23, 2014·International Journal of Epidemiology·Carmen M Martin-RuizThomas von Zglinicki
Oct 9, 2014·International Journal of Clinical Pharmacology and Therapeutics·Birte HofmannChristian Zurth
Nov 20, 2014·PloS One·Fernanda Gutierrez-RodriguesRodrigo T Calado
Dec 6, 2014·BMC Research Notes·Joshua DenhamFadi J Charchar

❮ Previous
Next ❯

Citations

Oct 28, 2016·Molecular Ecology·Paul V DebesAnti Vasemägi
Jan 26, 2017·American Journal of Human Biology : the Official Journal of the Human Biology Council·Dan T A EisenbergChristopher W Kuzawa
Oct 27, 2015·European Journal of Nutrition·Hilary J BethancourtDaniel T A Eisenberg
Nov 24, 2016·International Journal of Epidemiology·Simon VerhulstAbraham Aviv
Mar 13, 2018·American Journal of Human Biology : the Official Journal of the Human Biology Council·Farzana JasmineMuhammad G Kibriya
Aug 15, 2017·International Journal of Molecular Sciences·Payal P KhinchaShahinaz M Gadalla
Apr 7, 2018·Molecular Biology Reports·Karen M Jiménez, Diego A Forero
Jun 28, 2016·Gene Regulation and Systems Biology·Lilit Nersisyan
Aug 22, 2018·American Journal of Human Biology : the Official Journal of the Human Biology Council·Robert L TennysonDan T A Eisenberg
Dec 13, 2018·Annals of the American Thoracic Society·Andrew M Courtwright, Souheil El-Chemaly
May 30, 2019·Proceedings. Biological Sciences·Dan T A EisenbergChristopher W Kuzawa
Jan 18, 2018·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Dan T A Eisenberg, Christopher W Kuzawa
Aug 6, 2019·American Journal of Human Biology : the Official Journal of the Human Biology Council·Erin E MastersonDan T A Eisenberg
Dec 18, 2019·American Journal of Physical Anthropology·Dan T A EisenbergChristopher W Kuzawa
Nov 5, 2019·Molecular Ecology Resources·Simon Verhulst
Mar 7, 2020·Molecular Ecology Resources·Francisco MorinhaGuillermo Blanco
Aug 26, 2018·Scientific Reports·Luise A SeekerGeorgios Banos
Apr 22, 2019·European Journal of Nutrition·Kerry S FlannaganEduardo Villamor
May 3, 2019·PloS One·Daniel NettleMelissa Bateson
Aug 15, 2017·Molecular Ecology Resources·Justin R EastwoodAnne Peters
May 8, 2019·AJR. American Journal of Roentgenology·Vladimir I ValtchinovAli S Raja
Sep 12, 2020·Metabolites·Fábio Renato Manzolli LeiteRodrigo López
Sep 28, 2017·American Journal of Human Biology : the Official Journal of the Human Biology Council·Elisabeth A GoldmanKirstin N Sterner
Dec 11, 2020·American Journal of Physical Anthropology·Tiffany D PanDan T A Eisenberg
Feb 16, 2021·Molecular Ecology·Alexandra M SparksHannah L Dugdale
Jun 30, 2019·Journal of the American Society for Mass Spectrometry·Mirjam VisscherGijs Van Soest
Jul 18, 2021·Environment International·Shohreh F FarzanMaria Argos
Aug 5, 2021·Journal of Clinical Laboratory Analysis·Guozhu SunJun-Hua Xiao
Sep 18, 2021·Journal of the American Geriatrics Society·Robert J Pignolo, F Brad Johnson
Oct 3, 2021·Journal of Assisted Reproduction and Genetics·Mette W ChristensenHans Jakob Ingerslev
Aug 27, 2021·Molecular Ecology·Florentin RemotJean-François Lemaitre

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

American Journal of Human Biology : the Official Journal of the Human Biology Council
Monika KrzyżanowskaJean-Christophe Thalabard
American Journal of Human Biology : the Official Journal of the Human Biology Council
Amy L Non, Zaneta M Thayer
© 2022 Meta ULC. All rights reserved