Improving results in rat fracture models: enhancing the efficacy of biomechanical testing by a modification of the experimental setup

BMC Musculoskeletal Disorders
Peter Michael ProdingerThomas Tischer

Abstract

Animal fracture models, primarily performed in rats, are crucial to investigate normal and pathological bone healing. However, results of biomechanical testing representing a major outcome measure show high standard deviations often precluding statistical significance. Therefore, the aim of our study was a systematical examination of biomechanical characteristics of rat femurs during three-point bending. Furthermore, we tried to reduce variation of results by individually adapting the span of bearing and loading areas to the bone's length. We examined 40 paired femurs of male Wistar-rats by DXA (BMD and BMC of the whole femur) and pQCT-scans at the levels of bearing and loading areas of the subsequent biomechanical three-point bending test. Individual adjustment of bearing and loading bars was done respecting the length of each specimen. Subgroups of light (< 400 g, n = 22) and heavy (> 400 g, n = 18) animals were formed and analysed separately. We furthermore compared the results of the individualised bending-setting to 20 femurs tested with a fix span of 15 mm. Femurs showed a length range of 34 to 46 mm. The failure loads ranged from 116 to 251 N (mean 175.4 ± 45.2 N; heavy animals mean 221 ± 18.9 N; light animals mean 138.1...Continue Reading

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Citations

Aug 28, 2020·Gut Microbes·Mirna ČokloSandra Kraljević Pavelić
Nov 19, 2020·Brazilian Oral Research·Luis Gustavo Gonzalez OsunaPriscilla Barbosa Ferreira Soares
Feb 9, 2021·The British Journal of Nutrition·Alexandros S ZervasIsmene A Dontas
Jun 22, 2021·Clinical and Experimental Pharmacology & Physiology·Tamer ErenerÖzge Sezgin
Nov 26, 2021·Frontiers in Bioengineering and Biotechnology·Philip ProcterSune Larsson

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Methods Mentioned

BETA
imaging technique
X-ray

Software Mentioned

GraphPad Prism
View
GraphPad
Scout

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