Improving Saccharomyces cerevisiae ethanol production and tolerance via RNA polymerase II subunit Rpb7

Biotechnology for Biofuels
Zilong Qiu, Rongrong Jiang

Abstract

Classical strain engineering methods often have limitations in altering multigenetic cellular phenotypes. Here we try to improve Saccharomyces cerevisiae ethanol tolerance and productivity by reprogramming its transcription profile through rewiring its key transcription component RNA polymerase II (RNAP II), which plays a central role in synthesizing mRNAs. This is the first report on using directed evolution method to engineer RNAP II to alter S. cerevisiae strain phenotypes. Error-prone PCR was employed to engineer the subunit Rpb7 of RNAP II to improve yeast ethanol tolerance and production. Based on previous studies and the presumption that improved ethanol resistance would lead to enhanced ethanol production, we first isolated variant M1 with much improved resistance towards 8 and 10% ethanol. The ethanol titers of M1 was ~122 g/L (96.58% of the theoretical yield) under laboratory very high gravity (VHG) fermentation, 40% increase as compared to the control. DNA microarray assay showed that 369 genes had differential expression in M1 after 12 h VHG fermentation, which are involved in glycolysis, alcoholic fermentation, oxidative stress response, etc. This is the first study to demonstrate the possibility of engineering euk...Continue Reading

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Citations

Jun 12, 2020·Biotechnology for Biofuels·Vijayendran RaghavendranJeffrey Green
Oct 20, 2018·Frontiers in Bioengineering and Biotechnology·Leonardo Martins-SantanaRafael Silva-Rocha
Aug 18, 2020·International Journal of Food Microbiology·Ahasanul KarimMohammed Aïder
Jan 9, 2019·Biochemistry·Zihe LiuJens Nielsen

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Datasets Mentioned

BETA
GSE77853

Methods Mentioned

BETA
PCR
reverse transcription PCR

Software Mentioned

Agilent Genespring GX
gTME

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