Improving Senescent Wound Healing With Local and Systemic Therapies

Annals of Plastic Surgery
Caroline SzpalskiStephen M Warren

Abstract

The population is aging, and the prevalence of chronic wounds is increasing. Because neovascularization is essential for tissue repair and both local and systemic factors affect new blood vessel formation, we hypothesize that altering either pathway would reciprocally enhance wound healing in the aged. To test this hypothesis, p53 was locally suppressed and endothelial progenitor cells (EPCs) were systemically mobilized in a murine model of senescent wound healing.Bilateral 6-mm full-thickness stented wounds were made on the dorsum of Zmpste24 mice. Animals received weekly topical p53 small interfering RNA (siRNA) (n = 25), weekly topical nonsense siRNA (n = 25), daily subcutaneous AMD3100 injections (n = 25), or daily subcutaneous saline injections (n = 25). Wounds were photographically assessed and harvested for reverse transcription polymerase chain reaction, enzyme-linked immunosorbent assay, and immunostaining over 40 days. Circulating EPC levels were measured using fluorescence-activated cell sorting analysis.Local p53 siRNA significantly improved Zmpste24 wound healing (18 ± 2 vs 40 ± 3 days; P ≤ 0.0001). p53 siRNA significantly increased local provasculogenic factors (hypoxia-inducible factor 1 α, stromal cell-derived f...Continue Reading

References

Jul 2, 1992·Nature·D P Lane
Aug 1, 1990·Biological Reviews of the Cambridge Philosophical Society·Z A Medvedev
May 1, 1997·Journal of Wound Care·H Desai
Dec 28, 1999·Annual Review of Cell and Developmental Biology·G L Semenza
Jan 3, 2003·Biogerontology·Gillian S AshcroftJason J Ashworth
Feb 13, 2004·World Journal of Surgery·Ankush Gosain, Luisa A DiPietro
Jul 21, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Robert D GalianoGeoffrey C Gurtner
Aug 17, 2004·American Journal of Physiology. Cell Physiology·Takayuki Asahara, Atsuhiko Kawamoto
Jun 28, 2005·Nature Medicine·Baohua LiuZhongjun Zhou
Dec 8, 2005·Nature Medicine·Karim Bensaad, Karen H Vousden
Feb 6, 2007·Clinics in Dermatology·Nathan B MenkeRobert F Diegelmann
Jul 10, 2007·Lancet·José A Esté, Amalio Telenti
Aug 2, 2007·Journal of Wound, Ostomy, and Continence Nursing : Official Publication of the Wound, Ostomy and Continence Nurses Society·Joyce Pittman
Feb 26, 2008·Cell·Diana J LairdAmy J Wagers
Feb 21, 2009·Plastic and Reconstructive Surgery·Shang A LohGeoffrey C Gurtner
Nov 28, 2009·Experimental & Molecular Medicine·Li ChenFutoshi Shibasaki
Mar 13, 2010·Annals of the Royal College of Surgeons of England·T IbrahimW M Harper
Oct 20, 2010·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Phuong D NguyenPierre B Saadeh
Nov 30, 2012·Plastic and Reconstructive Surgery·Parag ButalaStephen M Warren
Jan 9, 2013·Methods in Molecular Biology·Yingjuan Qian, Xinbin Chen
Apr 17, 2013·Cell Cycle·Morvarid Farhang GhahremaniJody J Haigh
Mar 22, 2014·PloS One·Robert J AllenStephen M Warren

❮ Previous
Next ❯

Citations

Aug 10, 2021·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Elayaraja KolanthaiSudipta Seal

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Auditory Perception

Auditory perception is the ability to receive and interpret information attained by the ears. Here is the latest research on factors and underlying mechanisms that influence auditory perception.

Related Papers

The Journal of Investigative Dermatology
Yukihide NishimuraDouglas W Losordo
Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Lee M MorrisTimothy M Crombleholme
Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Jihyun UmKi-Sook Park
© 2022 Meta ULC. All rights reserved