Improving survival and storage stability of bacteria recalcitrant to freeze-drying: a coordinated study by European culture collections

Applied Microbiology and Biotechnology
Jindrich PeirenDavid R Arahal

Abstract

The objective of this study is to improve the viability after freeze-drying and during storage of delicate or recalcitrant strains safeguarded at biological resource centers. To achieve this objective, a joint experimental strategy was established among the different involved partner collections of the EMbaRC project ( www.embarc.eu ). Five bacterial strains considered as recalcitrant to freeze-drying were subjected to a standardized freeze-drying protocol and to seven agreed protocol variants. Viability of these strains was determined before and after freeze-drying (within 1 week, after 6 and 12 months, and after accelerated storage) for each of the protocols. Furthermore, strains were exchanged between partners to perform experiments with different freeze-dryer-dependent parameters. Of all tested variables, choice of the lyoprotectant had the biggest impact on viability after freeze-drying and during storage. For nearly all tested strains, skim milk as lyoprotectant resulted in lowest viability after freeze-drying and storage. On the other hand, best freeze-drying and storage conditions were strain and device dependent. For Aeromonas salmonicida CECT 894(T), best survival was obtained when horse serum supplemented with trehal...Continue Reading

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Citations

Jun 25, 2016·World Journal of Microbiology & Biotechnology·Yan-Zhen MeiWen-Wan Fang
Mar 21, 2018·International Journal of Systematic and Evolutionary Microbiology·Kenta Sakurai, Hiroko Kawasaki
Dec 6, 2017·Microbial Biotechnology·Teresa BerningerAngela Sessitsch

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