Abstract
In order to improve the efficacy of targeted therapy trials, the expression profiles of several molecular markers that are potential candidates for targeted therapy were analyzed in patients with progressive castration-resistant prostate cancer. Paraffin-embedded samples of tumor tissue from 51 patients obtained from biopsies of metastases or remaining prostates were analyzed immunohistochemically for the expression of EGFR, PDGFRβ, Her-2/neu, c-Kit, and VEGF. Staining was analyzed according to the percentage of positively stained tumor cells and the intensity of staining. According to the different cut-off values of 10%, 30%, 50%, or 70% for the percentage of positively stained cells, different rates of expression were found. Expression rates ranged from 30.6% to 61.2% for EGFR, from 34.7% to 57.1% for PDGFRβ, from 9.6% to 28.8% for Her-2/neu, from 12.5% to 22.4% for c-Kit, and from 51.1% to 74.5% for VEGF. Defining positive expression as ≥ 30% positively stained tumor cells, with an intensity of staining of ≥ 2+, resulted in positive expression of EGFR in 38.8%, PDGFRβ in 24.5%, Her-2/neu in 13.5%, c-Kit in 6.4%, and VEGF in 44.7% of the patients. Our results demonstrate simultaneous expression of several markers in castratio...Continue Reading
References
Dec 7, 2000·Journal of the National Cancer Institute·S SignorettiM Loda
May 20, 2003·Journal of Neurology, Neurosurgery, and Psychiatry·A M StarkH M Mehdorn
May 13, 2004·Cancer·Primo N LaraDavid R Gandara
Jul 21, 2004·The Prostate·Ali ZiadaE David Crawford
Sep 25, 2004·The Prostate·Kamakshi RaoRobert S DiPaola
Nov 19, 2004·Neoplasia : an International Journal for Oncology Research·Matthias D HoferMark A Rubin
Feb 8, 2005·Seminars in Oncology·Lee M Ellis
May 24, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Daniel VallböhmerHeinz-Josef Lenz
Nov 2, 2005·Pathology, Research and Practice·Guven AslanIlhan Celebi
May 26, 2006·The American Journal of Surgical Pathology·Jorge L YaoP Anthony di Sant'Agnese
Jun 27, 2006·BJU International·Amy M LinEric J Small
Jan 20, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Johann Sebastian de BonoJohn Graham
Mar 22, 2007·Molecular Cancer Research : MCR·Josep Tabernero
Apr 15, 2008·The Prostate·Rebecca A MarksLiang Cheng
May 10, 2008·Annals of Oncology : Official Journal of the European Society for Medical Oncology·G GravisP Viens
Jul 17, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Antje HoeringJohn J Crowley
Jul 17, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Boris FreidlinAlison Martin
Oct 14, 2008·Oncogene·M R RussellA Fatatis
May 9, 2009·International Journal of Molecular Medicine·M L Dos SantosM A Nagai
Citations
Aug 27, 2011·BMC Cancer·Steffen WedelRoman A Blaheta
Jul 16, 2011·Actas urologicas españolas·N MottetA Heidenreich
Feb 15, 2011·European Urology·Nicolas MottetAxel Heidenreich