Improving the efficiency of enzyme utilization for sugar beet pulp hydrolysis

Bioprocess and Biosystems Engineering
Yi ZhengJean Vandergheynst

Abstract

Sugar beet pulp (SBP) is a carbohydrate-rich residue of table sugar processing. It shows promise as a feedstock for fermentable sugar and biofuel production via enzymatic hydrolysis and microbial fermentation. This research focused on the enzymatic hydrolysis of SBP and examined the effects of solid loading (2-10 %, dry basis), enzyme preparation, and enzyme recycle on the production of fermentable sugars. The enzyme partitioning to the solid and liquid phases during SBP enzymatic hydrolysis and loss during recycling were investigated using SDS-PAGE and Zymogram analysis. Without considering product inhibition, the cellulase added initially to the SBP hydrolysis lost only 6 % filter paper activity and negligible carboxymethyl cellulose activity upon multiple cycles of SBP hydrolysis. It was found that enzyme dosage can be reduced by 50 % while maintaining similar, and in some cases higher fermentable sugar yield. The removal of hydrolysis products will further improve enzymatic hydrolysis of SBP for biofuel production.

References

Jun 13, 2000·Applied Biochemistry and Biotechnology·J B DoranB Foster
Apr 20, 2002·Applied Biochemistry and Biotechnology·B L FosterJ B Doran-Peterson
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Sep 21, 2007·Chemistry Central Journal·Sohel DalalMunishwar Nath Gupta

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Citations

Jan 1, 2016·Bioprocess and Biosystems Engineering·Pei-Jun LiYang Shan

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