Improving the osteogenesis of rat mesenchymal stem cells by chitosan-based-microRNA nanoparticles

Carbohydrate Polymers
Guangsheng WuXiguang Chen

Abstract

MicroRNAs (miRNAs) play important roles in the osteogenic differentiation of stem cells. However, the application of miRNA in bone regeneration has been limited by its poor stability, low cellular uptake, and undesired immune response. In this study, chitosan (CS)/tripolyphosphate (TPP)/Hyaluronic Acid (HA) nanoparticles (CTH NPs) were prepared to deliver antimiR-138 to bone marrow mesenchymal stem cells (MSCs). The particle size, polydispersity index, and zeta potential of CTH NPs were related to the weight ratio of CS:TPP:HA. At optimum N/P ratio (20:1), the highest encapsulation efficiency was obtained. Both blank CTH NPs and CTH/antmiR-138 NPs exhibited no cytotoxicity to MSCs. A high transfection efficiency (nearly 70%) and significant enhancement of the osteogenesis of MSCs were observed. Above results demonstrated that CTH NPs was a potential candidate as an efficient non-viral miRNA vector to regulate the osteogenic differentiation of MSCs.

References

Oct 20, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sunil A AgnihotriTejraj M Aminabhavi
May 2, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tomoko ItoYoshiyuki Koyama
Jul 16, 2009·Journal of Cellular and Molecular Medicine·Neeraj Kumar SatijaG U Gurudutta
Aug 25, 2010·International Journal of Pharmaceutics·Walter E Rudzinski, Tejraj M Aminabhavi
Mar 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Tilde EskildsenMoustapha Kassem
Aug 27, 2011·Expert Opinion on Drug Delivery·Kiran ChaturvediTejraj M Aminabhavi
Sep 14, 2011·International Journal of Pharmaceutics·Hua-Ding LuLu-Lu Lv
Nov 29, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jostein MalmoSabina P Strand
Jan 31, 2012·Chemistry & Biology·John C Burnett, John J Rossi
Jul 17, 2012·Journal of Prosthodontic Research·Hiroshi EgusaKentaro Akiyama
Jan 1, 2013·Colloids and Surfaces. B, Biointerfaces·Sonia Al-QadiCarmen Remunán-López
Aug 24, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Héloïse RagelleVéronique Préat
Oct 1, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yu ZhangRichard A Gemeinhart
Dec 30, 2014·International Journal of Biological Macromolecules·M SriramN Selvamurugan
Jan 3, 2015·Advanced Drug Delivery Reviews·Kelsey R BeaversCraig L Duvall
Mar 3, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Yu-Bin MengXian-Jin Yang
Mar 11, 2015·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J R CostaM Pintado
Mar 15, 2015·Colloids and Surfaces. B, Biointerfaces·Chao FengXi Guang Chen

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Citations

Nov 1, 2016·Biotechnology Journal·Ahmed Abdal DayemSsang-Goo Cho
May 16, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zhonglong LiuYue He
Nov 14, 2019·Neurochemical Research·Vajihe AsgariHamid Bahramian
Jul 31, 2018·Frontiers in Bioengineering and Biotechnology·Celeste PiottoMikaël M Martino
Apr 10, 2019·International Journal of Endocrinology·Xi SunXianghang Luo
Nov 2, 2019·Nanomedicine : Nanotechnology, Biology, and Medicine·Fusong JiangXiaowei Yu
Dec 17, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Farah Asa'adLena Larsson
Jul 30, 2021·Advanced Drug Delivery Reviews·Yaping Zhuang, Wenguo Cui
Jul 10, 2017·ACS Biomaterials Science & Engineering·Eleni K TsekouraHasan Uludag
May 22, 2020·Current Stem Cell Research & Therapy·Tianxu ZhangRonghui Zhou

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