Improving the visibility of tooth cementum annulations by adjustment of the cutting angle of microscopic sections

Forensic Science International : Synergy
George J R MaatM J Aarents

Abstract

Age at death assessments by counting tooth cementum annulations (TCAs) in unstained undecalcified microscopic ground sections of (single rooted) teeth is, amongst others, problematic because of the unclear distinction between the bright and darker annulations. Counting is hampered by optical superimposition of the tangentially positioned layers of cementum in the section since 'regular transverse sections' run perpendicular to the axis of a cone-shaped root with its yearly deposited cone-shaped layers of cementum. This study demonstrates that to improve the visibility of the annulations, the cutting angle should be perpendicular to the exterior of a root, not perpendicular to its axis. The site where the cut hits the root perpendicular should show the best possible distinction between the TCAs. Here, superimposition of the now vertical positioned layers within the section will result in increased contrast between bright and darker layers. A procedure for such preparation is given.

References

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Citations

Jun 29, 2013·Forensic Science International : Synergy·Ademir FrancoGuy Willems
Nov 2, 2013·Homo : internationale Zeitschrift für die vergleichende Forschung am Menschen·E ZorbaK Moraitis
Dec 12, 2012·Homo : internationale Zeitschrift für die vergleichende Forschung am Menschen·J Gauthier, H Schutkowski
Jul 30, 2009·American Journal of Physical Anthropology·Mirjana RoksandicDiana Voicu
Nov 28, 2012·Journal of Forensic Sciences·Timothy P Gocha, Holger Schutkowski
Sep 4, 2016·International Journal of Legal Medicine·Inês Oliveira-SantosDavid Gonçalves

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