IMS-IMS and IMS-IMS-IMS/MS for separating peptide and protein fragment ions

Analytical Chemistry
Samuel I MerenbloomD E Clemmer

Abstract

Multidimensional ion mobility spectrometry (IMS-IMS and IMS-IMS-IMS) techniques have been combined with mass spectrometry (MS) and investigated as a means of generating and separating peptide and protein fragment ions. When fragments are generated inside a drift tube and then dispersed by IMS prior to MS analysis, it is possible to observe many features that are not apparent from MS analysis alone. The approach is demonstrated by examining fragmentation patterns arising from electrospray ion distributions of insulin chain B and ubiquitin. The multidimensional IMS approach makes it possible to select individual components for collisional activation and to disperse fragments based on differences in mobility prior to MS analysis. Such an approach makes it possible to observe many features not apparent by MS analysis alone.

References

Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·W J HenzelC Watanabe
Oct 30, 1998·Journal of the American Society for Mass Spectrometry·S J ValentineD E Clemmer
Apr 10, 2002·Analytical and Bioanalytical Chemistry·D C Collins, M L Lee

❮ Previous
Next ❯

Citations

Mar 6, 2008·Analytical and Bioanalytical Chemistry·Larissa S Fenn, John A McLean
Feb 28, 2009·Analytical and Bioanalytical Chemistry·Larissa S FennJohn A McLean
Jan 13, 2011·Analytical and Bioanalytical Chemistry·András Kiss, Ron M A Heeren
Oct 11, 2011·International Journal of Health Care Finance and Economics·Aysegul TimurJeffrey DeSimone
Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Stephen J ValentineDavid E Clemmer
Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Zhiyu LiDavid E Clemmer
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Alexandre A ShvartsburgThomas O Metz
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Steven M ZuckerDavid E Clemmer
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Ruwan T KurulugamaDavid E Clemmer
Mar 21, 2013·Journal of the American Society for Mass Spectrometry·Jonathan M DilgerDavid E Clemmer
Nov 7, 2006·Journal of the American Society for Mass Spectrometry·Stormy L Koeniger, David E Clemmer
May 22, 2007·Journal of the American Society for Mass Spectrometry·Erin Shammel BakerRichard D Smith
Jan 30, 2008·Journal of the American Society for Mass Spectrometry·Erin Shammel BakerRichard D Smith
Mar 4, 2008·Journal of the American Society for Mass Spectrometry·Isabel Riba-GarciaSimon J Gaskell
Sep 2, 2008·Journal of the American Society for Mass Spectrometry·Manolo D PlasenciaDavid E Clemmer
Jun 6, 2009·Journal of the American Society for Mass Spectrometry·Qin ZhaoEthan R Badman
Jul 8, 2010·Journal of the American Society for Mass Spectrometry·Jaakko LaakiaTapio Kotiaho
Jan 16, 2007·Analytical Chemistry·Samuel I MerenbloomDavid E Clemmer
Mar 4, 2008·Analytical Chemistry·Brian H ClowersRichard D Smith
Jun 28, 2008·Analytical Chemistry·Mikhail E BelovRichard D Smith
Jan 16, 2009·Analytical Chemistry·Samuel I MerenbloomDavid E Clemmer
Sep 30, 2009·Analytical Chemistry·Satendra PrasadRichard D Smith
Aug 31, 2006·Journal of the American Chemical Society·Stormy L KoenigerDavid E Clemmer
Jan 25, 2008·The Journal of Physical Chemistry. a·Nick C PolferDavid E Clemmer
May 18, 2010·The Journal of Physical Chemistry. B·Nicholas A PiersonDavid E Clemmer
Jun 7, 2012·The Journal of Physical Chemistry. B·Natalya AtlasevichDavid E Clemmer
Feb 2, 2008·Journal of Proteome Research·D IsailovicD E Clemmer
Jul 19, 2008·Annual Review of Analytical Chemistry·Brian C BohrerDavid E Clemmer
Sep 5, 2012·Genome Medicine·Erin Shammel BakerRichard D Smith
Feb 25, 2006·AJR. American Journal of Roentgenology·Diane K Wysowski, Parivash Nourjah
May 30, 2015·International Journal of Mass Spectrometry·Zhiyu LiDavid E Clemmer
Jun 14, 2014·Journal of the American Society for Mass Spectrometry·Carlos LarribaDavid E Clemmer
Jul 12, 2014·Annual Review of Analytical Chemistry·Zhaorui ZhangLjiljana Paša-Tolić
Mar 25, 2014·Journal of the American Society for Mass Spectrometry·Huilin ShiDavid E Clemmer
Feb 5, 2016·Analytical Chemistry·Nicole Andrea MeyerGuillermo Vidal-de-Miguel
Jul 1, 2008·Expert Review of Clinical Pharmacology·Steven Simoens
Feb 23, 2008·Analytical Chemistry·Samuel I MerenbloomDavid E Clemmer
Nov 26, 2015·Journal of Chromatography. a·Michael A EwingDavid E Clemmer
Nov 30, 2010·International Journal of Mass Spectrometry·Prabha DwivediHerbert H Hill
Dec 20, 2014·Analytical Chemistry·Jody C May, John A McLean
Jan 18, 2008·Journal of Mass Spectrometry : JMS·Abu B KanuHerbert H Hill
Oct 23, 2010·Journal of Mass Spectrometry : JMS·Prabha DwivediHerbert H Hill
Apr 25, 2009·Mass Spectrometry Reviews·Ryan T KellyRichard D Smith

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.