Imsnc761 and DDX6 synergistically suppress cell proliferation and promote apoptosis via p53 in testicular embryonal carcinoma cells

Bioscience Reports
Zhengzheng DuanF Sun

Abstract

Intermediate-sized non-coding RNAs (imsncRNAs) have been shown to play important regulatory roles in the development of several eukaryotic organisms. In the present research, we selected imsncRNA 761 (imsnc761) as a research target. Expression analyses in a previous study showed that imsnc761 was down-regulated in maturation-arrested testis tissues as compared with the level in normal controls. In the present study, we found that imsnc761 could interact with DEAD-box helicase 6 (DDX6) to induce NTERA-2 (NT2 (testicular embryonal carcinoma cell)) cell apoptosis and proliferation inhibition via the p53 pathway. This interaction between imsnc761 and DDX6 also inhibited mitochondrial function and specific gene transcription and translation. To facilitate further research, we used label-free quantitation method to analyze the associated differences in Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathways and biological processes. This confirmed the changes in several specific pathways, which matched our molecular experimental results.

References

Jul 19, 2000·Archives of Biochemistry and Biophysics·A Abou-Haila, D R Tulsiani
Jun 1, 2005·Gene·Antonella Spinazzola, Massimo Zeviani
Nov 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Seong-Woon YuValina L Dawson
Jan 30, 2008·Gene·Fabrício F Costa
Nov 3, 2009·Trends in Cell Biology·Daniel Speidel
Sep 17, 2010·Journal of Cell Science·Shawn B Bratton, Guy S Salvesen
Oct 12, 2010·RNA Biology·Tiffany Hung, Howard Y Chang
Nov 6, 2012·Gene·Shen-Hsi YangAlan J Whitmarsh
Dec 15, 2012·Science·Jeannie T Lee
Jan 29, 2013·RNA Biology·Frances V Fuller-Pace
Mar 2, 2013·Cell Death and Differentiation·M BohgakiR Hakem
Mar 27, 2013·Biochimica Et Biophysica Acta·Vlad Presnyak, Jeff Coller
Apr 9, 2013·Gene·Mohammed TariqueRenu Tuteja
Oct 22, 2013·Histochemistry and Cell Biology·Chika KawaharaHideaki Fujita
Apr 18, 2014·Epigenetics : Official Journal of the DNA Methylation Society·Veronica J Peschansky, Claes Wahlestedt
May 3, 2014·Wiley Interdisciplinary Reviews. RNA·Dirk H OstareckAntje Ostareck-Lederer
Feb 28, 2015·Nucleic Acids Research·Sarah NicklasJens C Schwamborn
Apr 15, 2015·Trends in Genetics : TIG·Georges St LaurentPhilipp Kapranov
Apr 24, 2015·Handbook of Experimental Pharmacology·Nassim Dali-Youcef, Romeo Ricci
Nov 27, 2015·The FEBS Journal·Evangelos Giampazolias, Stephen W G Tait
Dec 17, 2015·Nature Reviews. Genetics·Jeffrey J Quinn, Howard Y Chang
Jan 28, 2016·Journal of Diabetes Investigation·Tetsuya Hosooka, Wataru Ogawa
Apr 15, 2016·Blood Reviews·Eduardo FuentesIván Palomo
May 14, 2016·Nucleic Acids Research·Hadi JorjaniAndreas R Gruber
Jul 10, 2016·Archives of Medical Research·Shuyun YangZhixian He
Jun 16, 2017·Journal of Biomedical Science·Chantal Hoi Yin Cheung, Hsueh-Fen Juan
Jul 19, 2017·Proteomes·Evelyn Ramberger, Gunnar Dittmar
Jul 27, 2017·Protoplasma·Marina Klemenčič, Christiane Funk
Aug 2, 2017·Mass Spectrometry Reviews·Francisco Calderón-CelisAlfredo Sanz-Medel
Aug 16, 2017·Seminars in Cell & Developmental Biology·Vincent BoivinMichelle S Scott

❮ Previous
Next ❯

Citations

Apr 4, 2021·International Journal of Molecular Sciences·Olga Sergeeva, Timofei Zatsepin

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
PCR
Transfection
immunoprecipitation
pull-down
flow cytometry
Assay
in vitro transcription
RIP
flow

Software Mentioned

Ensembl

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis