In benign Barrett's epithelial cells, acid exposure generates reactive oxygen species that cause DNA double-strand breaks.

Cancer Research
Hui Ying ZhangRhonda F Souza

Abstract

Cells that sustain double-strand breaks (DSB) can develop genomic instability, which contributes to carcinogenesis, and agents that cause DSBs are considered potential carcinogens. We looked for evidence of acid-induced DNA damage, including DSBs, in benign Barrett's epithelial (BAR-T) cell lines in vitro and in patients with Barrett's esophagus in vivo. In BAR-T cells, we also explored the mechanisms underlying acid-induced DNA damage. We exposed BAR-T cells to acid in the presence of a fluorescent probe for reactive oxygen species (ROS) and in the presence or absence of disodium 4,4'-diisothiocyanatostilbene-2,2'-disulfonate (which prevents intracellular acidification) and N-acety-l-cysteine (a scavenger of ROS). DSBs were detected by Western blotting and immunofluorescence for histone H2AX phosphorylation and by CometAssay. During endoscopy in patients with Barrett's esophagus, we took biopsy specimens from the metaplastic mucosa before and after esophageal perfusion with 0.1 N HCl for 3 min and sought DSBs by Western blotting for histone H2AX phosphorylation. In BAR-T cells, acid exposure resulted in ROS production and caused a time-dependent increase in levels of phospho-H2AX that continued for at least 48 h. Pretreatment ...Continue Reading

References

Aug 1, 1992·Mutation Research·T MoritaK Okumura
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P L BlountB J Reid
Aug 1, 1997·The Journal of Biological Chemistry·E S Henle, S Linn
Apr 16, 1998·The Journal of Biological Chemistry·E P RogakouW M Bonner
Dec 3, 1999·Science·T Lindahl, R D Wood
Dec 2, 2000·Nature·B B Zhou, S J Elledge
Feb 24, 2001·Alimentary Pharmacology & Therapeutics·R C FitzgeraldG Triadafilopoulos
Feb 27, 2001·FEBS Letters·J Bartek, J Lukas
Sep 19, 2002·Radiation Research·Olga A SedelnikovaWilliam M Bonner
Apr 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Hai XiaoLeroy F Liu
Jul 9, 2003·Immunological Reviews·Kevin D MillsFrederick W Alt
Jun 16, 2004·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·J R OlliverC P Wild
Jan 20, 2005·Journal of the National Cancer Institute·Heiko Pohl, H Gilbert Welch
Mar 16, 2005·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Joanna R OlliverChristopher P Wild
Apr 20, 2005·Oncogene·Sandra L Harris, Arnold J Levine
Aug 29, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kathy Hormi-CarverRhonda F Souza
Feb 3, 2007·The American Journal of Gastroenterology·Linda A FeaginsRhonda F Souza
May 19, 2007·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·K R JaiswalR F Souza
Dec 8, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Linda A FeaginsRhonda F Souza
Feb 21, 2008·The EMBO Journal·Razqallah Hakem
Oct 9, 2008·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·P Lao-SirieixR C Fitzgerald
Nov 14, 2008·Nature Reviews. Cancer·William M BonnerYves Pommier
Mar 10, 2009·Methods : a Companion to Methods in Enzymology·Wenjuan LiaoWei-Guo Zhu

❮ Previous
Next ❯

Citations

Oct 18, 1994·Biochimica Et Biophysica Acta·A FlynnA Kaminski
Jun 15, 2012·Nature Reviews. Cancer·Robert J GilliesRobert A Gatenby
Sep 6, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Elena ZaikaAlexander I Zaika
Jun 4, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xiaofang HuoRhonda F Souza
Jul 20, 2011·Case Reports in Gastroenterology·Yasuhiko AbeTooru Shimosegawa
Feb 12, 2014·Antioxidants & Redox Signaling·Valery V Khramtsov, Robert J Gillies
Apr 6, 2012·Cellular Oncology (Dordrecht)·P BusJ W P M van Baal
Jan 19, 2010·Gastroenterology·Stuart Jon SpechlerKenneth K Wang
May 26, 2015·Current Treatment Options in Rheumatology·Vivek NagarajaDinesh Khanna
Oct 21, 2016·The Journal of Pharmacology and Experimental Therapeutics·Dan LiWeibiao Cao
Apr 26, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Stine F PedersenLuis A Pardo
Oct 15, 2013·Annals of the New York Academy of Sciences·Yu FangKaterina Dvorak
Sep 10, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Hong ZhaoZbigniew Darzynkiewicz
Mar 27, 2019·Cancer Metastasis Reviews·Smitha R PillaiRobert J Gillies
Apr 8, 2014·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Arcadi Cipponi, David M Thomas
May 27, 2014·Expert Review of Gastroenterology & Hepatology·Katie E O'SullivanJohn V Reynolds
Sep 13, 2013·American Journal of Physiology. Cell Physiology·Jie HongWeibiao Cao
May 24, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sui PengStuart Jon Spechler
Nov 16, 2019·Annual Review of Physiology·Ebbe Boedtkjer, Stine F Pedersen
Jan 28, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xiaofang HuoRhonda F Souza
Apr 8, 2020·Cancer Metastasis Reviews·Myungjun KoRajini Rao
Sep 29, 2011·Annals of the New York Academy of Sciences·Gary W FalkGerardo Nardone
Aug 22, 2020·Annals of the New York Academy of Sciences·Carmelo ScarpignatoRichard H Hunt
Aug 28, 2020·Gastroenterology Report·Erik J SniderWilliam M Grady
Jan 11, 2019·Journal of Experimental & Clinical Cancer Research : CR·Zhangjian ZhouDunfa Peng
Apr 5, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Dan Li, Weibiao Cao
Mar 2, 2018·Cancer Research·Nadeem O Kaakoush
Nov 5, 2020·European Journal of Medicinal Chemistry·Gargi Nikhil VaidyaDinesh Kumar
Jun 4, 2019·Biochimica Et Biophysica Acta. Reviews on Cancer·Ravindran Caspa GokulanAlexander I Zaika
May 29, 2021·The Surgical Clinics of North America·Ekaterina Fedorova, Thomas J Watson
Jun 29, 2021·Frontiers in Genetics·Manisha BajpaiHana Aviv
Oct 5, 2021·Frontiers in Genetics·Anshuman PandaManisha Bajpai

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