In-Cell NMR of Intrinsically Disordered Proteins in Mammalian Cells.

Methods in Molecular Biology
Juan A GerezRoland Riek

Abstract

In-cell NMR enables structural insights at atomic resolution of proteins in their natural environment. To date, very few methods have been developed to study proteins by in-cell NMR in mammalian systems. Here we describe a detailed protocol to conduct in-cell NMR on the intrinsically disordered protein of alpha-Synuclein (αSyn) in mammalian cells. This chapter includes a simplified expression and purification protocol of recombinant αSyn and its delivery into mammalian cells. The chapter also describes how to assess the cell leakage that might occur to the cells, the setup of the instrument, and how to perform basic analyses with the obtained NMR data.

Related Concepts

Related Feeds

Alpha-Synuclein Aggregation (MDS)

Alpha-synucleins are small proteins that are believed to restrict the mobility of synpatic vesicles and inhibit neurotransmitter release. Aggregation of these proteins have been linked to several types of neurodegenerative diseases including dementia with Lewy bodies and Parkinson's disease. Here is the latest research on α-synuclein aggregation.

Related Papers

Methods in Molecular Biology
Beata BekeiPhilipp Selenko
Chembiochem : a European Journal of Chemical Biology
Sina ReckelV Dötsch
Progress in Nuclear Magnetic Resonance Spectroscopy
Andres Y MaldonadoAlexander Shekhtman
Biochimica Et Biophysica Acta. General Subjects
Noritaka NishidaIchio Shimada
© 2021 Meta ULC. All rights reserved