In-channel electrochemical detection for microchip capillary electrophoresis using an electrically isolated potentiostat

Analytical Chemistry
R Scott MartinSusan M Lunte

Abstract

A new electrode configuration for microchip capillary electrophoresis (CE) with electrochemical (EC) detection is described. This approach makes it possible to place the working electrode directly in the separation channel. The "in-channel" EC detection was accomplished without the use of a decoupler through the utilization of a specially designed, electrically isolated potentiostat. The effect of the working electrode position on the separation performance (in terms of plate height and peak skew) of poly(dimethylsiloxane)-based microchip CEEC devices was evaluated by comparing the more commonly used end-channel configuration with this new in-channel approach. Using catechol as the test analyte, it was found that in-channel EC detection decreased the total plate height by a factor of 4.6 and lowered the peak skew by a factor of 1.3. A similar trend was observed for the small, inorganic ion nitrite. Furthermore, a fluorescent and electrochemically active amino acid derivative was used to directly compare the separation performance of in-channel EC detection to that of a widely used laser-induced fluorescence (LIF) detection scheme. In this case, it was found that the plate height and peak skew for both detection schemes were ess...Continue Reading

References

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Citations

Nov 15, 2002·Electrophoresis·Lisa A Holland, Alyison M Leigh
Nov 15, 2002·Electrophoresis·Walter R VandaveerSusan M Lunte
Feb 6, 2004·Journal of Chromatography. a·Chi-Chung Joseph LaiFu-Hsiang Ko
Sep 2, 2003·Analytical Chemistry·Damon M Osbourn, Craig E Lunte
Jun 25, 2009·Analytical Chemistry·Laura C Mecker, R Scott Martin
Mar 15, 2012·Analytical and Bioanalytical Chemistry·Dulan B GunasekaraJosé Alberto Fracassi da Silva
Apr 6, 2016·Analytical Chemistry·Lawrence P ZainoPaul W Bohn
Apr 5, 2015·Journal of Pharmaceutical and Biomedical Analysis·Nantana NuchtavornLeena Suntornsuk
May 13, 2015·Chemical Society Reviews·Darius G RackusAaron R Wheeler
Mar 25, 2005·BioTechniques·Gina S Fiorini, Daniel T Chiu
May 24, 2005·The Analyst·Leif Nyholm
Jun 2, 2007·Electrophoresis·Martin Pumera
Sep 12, 2007·Electrophoresis·Rongsheng ChenJieke Cheng
Nov 17, 2007·Electrophoresis·Zhi-Yong WuHubert H Girault
Nov 16, 2007·Electrophoresis·Mario Castaño-AlvarezAgustín Costa-García
Apr 4, 2008·Electrophoresis·Kyung-Sun LeeYoon-Bo Shim
Jul 29, 2010·Electrophoresis·Ana Fernández-la-VillaMario Castaño-Alvarez
Aug 16, 2005·Lab on a Chip·Lei JiangBingcheng Lin

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