In contrast to specific B cells, human basophils are unaffected by the toxic activity of an allergen toxin due to lack of internalization of immunoglobulin E-bound allergen

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
Daniel WickleinArnd Petersen

Abstract

Specific immunotherapy is the only curative therapy for type I allergies and the alarming increase in allergy prevalence emphasizes the need for additional/alternative strategies for curative treatment. Allergen toxins (AT), fusion products of an allergen with an apoptosis inducing cytotoxin, are a new kind of immunotoxin. AT should allow allergen-specific targeting and elimination of allergy-relevant cells, with B cells being the primary target. An important question is the fate of the effector cells, e.g. mast cells and basophils, which carry allergen-specific IgE: the immunotoxin might even prove to be harmful. We established a reliable in vitro B cell model (using two mouse hybridoma cell lines) for testing specificity and toxicity of P5-ETA', a fusion protein of the major timothy grass pollen allergen Phl p 5b and truncated Pseudomonas Exotoxin A. In a second step, we investigated the impact of the AT on human basophils. P5-ETA' reliably eliminated Phl p 5-specific cells in the in vitro B cell model, leaving unspecific B cells unharmed. Human basophils of grass pollen allergic donors specifically bound P5-ETA', released IL-4 and up-regulated the activation marker CD203c, but were not subject to the toxic effect because of ...Continue Reading

References

Sep 1, 1987·Immunopharmacology·H BoltanskyM Kaliner
Mar 1, 1994·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A PetersenM Schlaak
Apr 17, 1995·FEBS Letters·A BufeW M Becker
Jun 23, 1993·Annals of the New York Academy of Sciences·D Fitzgerald, I Pastan
Apr 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R J BinderP K Srivastava
Oct 26, 2001·Current Opinion in Immunology·J RingH Behrendt
Jun 25, 2002·Annals of Oncology : Official Journal of the European Society for Medical Oncology·R SchnellA Engert
Jan 18, 2003·The Journal of Allergy and Clinical Immunology·Joshua A Boyce
Feb 13, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R BoumizaJ Bienvenu
Mar 20, 2004·International Archives of Allergy and Immunology·Hans-Jörg BühringPeter Valent
Apr 22, 2004·The Journal of Allergy and Clinical Immunology·Harold S Nelson
May 20, 2004·International Journal of Medical Microbiology : IJMM·David J FitzGeraldIra Pastan
Jun 23, 2004·The Journal of Allergy and Clinical Immunology·Philip S Norman
Oct 14, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·B K LeeJ Y Ro
Oct 8, 2005·The Journal of Allergy and Clinical Immunology·Michael StöckerStefan Barth

❮ Previous
Next ❯

Citations

Feb 19, 2008·Journal of Neuroimmunology·Thomas NachreinerMichael Stöcker
Aug 30, 2012·Proteomics. Clinical Applications·Frederic BrosseronKatrin Marcus
Jan 16, 2016·Current Allergy and Asthma Reports·Robyn E O'HehirJennifer M Rolland
Feb 24, 2017·Toxins·Miriam OlombradaLucía García-Ortega

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Basophils

Basophils are myeloid cells with a high affinity IgE receptor and is involved in inflammatory responses during allergy. Discover the latest research on Basophils here.