In-depth structural analysis of glycans in the gas phase

Chemical Science
Eike MuchaKevin Pagel

Abstract

Although there have been substantial improvements in glycan analysis over the past decade, the lack of both high-resolution and high-throughput methods hampers progress in glycomics. This perspective article highlights the current developments of liquid chromatography, mass spectrometry, ion-mobility spectrometry and cryogenic IR spectroscopy for glycan analysis and gives a critical insight to their individual strengths and limitations. Moreover, we discuss a novel concept in which ion mobility-mass spectrometry and cryogenic IR spectroscopy is combined in a single instrument such that datasets consisting of m/z, collision cross sections and IR fingerprints can be obtained. This multidimensional data will then be compared to a comprehensive reference library of intact glycans and their fragments to accurately identify unknown glycans on a high-throughput scale with minimal sample requirements. Due to the complementarity of the obtained information, this novel approach is highly diagnostic and also suitable for the identification of larger glycans; however, the workflow and instrumentation is straightforward enough to be implemented into a user-friendly setup.

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Citations

Apr 25, 2020·Mass Spectrometry Reviews·David J Harvey
Jul 12, 2020·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Kim GreisKevin Pagel
Sep 6, 2019·Medicines·Patricia ReganMargaret Doherty
Dec 20, 2019·Analytical and Bioanalytical Chemistry·Maike LettowKevin Pagel
Sep 18, 2020·Chemistry : a European Journal·Chun-Wei ChangCheng-Chung Wang
Dec 23, 2020·Journal of the American Society for Mass Spectrometry·Abhigya MookherjeeMiklos Guttman
Jan 13, 2021·Current Opinion in Structural Biology·Roberta MarchettiAlba Silipo
Oct 24, 2020·Biochemistry·M Fernando BravoAdam B Braunschweig
Apr 24, 2021·Carbohydrate Research·Pia S Bruni, Stefan Schürch
May 7, 2021·Mass Spectrometry Reviews·Alba HykollariIain B H Wilson
May 29, 2021·Current Opinion in Biotechnology·Jerneja ŠtorNicole Borth
Mar 13, 2020·Chemical Science·Leonhard H UrnerKevin Pagel
Aug 14, 2019·Journal of the American Chemical Society·Christopher J GraySabine L Flitsch
Dec 28, 2019·The Journal of Physical Chemistry. B·Emvia I CalixteElyssia S Gallagher
May 23, 2020·Analytical Chemistry·Miloslav SandaRadoslav Goldman
Oct 6, 2021·Mass Spectrometry Reviews·Andreas ZappeKevin Pagel
Nov 5, 2021·Journal of the American Society for Mass Spectrometry·Emvia I CalixteElyssia S Gallagher

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Methods Mentioned

BETA
nuclear magnetic resonance
glycosylation
electrophoresis
NMR
infrared spectroscopy

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