In-droplet microparticle separation using travelling surface acoustic wave

Biomicrofluidics
Kwangseok ParkHyung Jin Sung

Abstract

Droplets in microfluidic systems can contain microscale objects such as cells and microparticles. The control of the positions of microscale objects within a microchannel is crucial for practical applications in not only continuous-flow-based but also droplet-based systems. This paper proposes an active method for the separation of microparticles inside moving droplets which uses travelling surface acoustic waves (TSAWs). We demonstrate the preconcentration and separation of 5 and 10 μm polystyrene microparticles in moving water-in-oil droplets through the application of TSAWs with two different frequencies. The microparticles inside the droplets are affected by the acoustic radiation force induced by the TSAWs to move laterally in the direction of the TSAW propagation and are thereby separated according to their size. In-droplet separation is then demonstrated through droplet splitting at a Y-junction. Compared to our previous studies, this acoustic approach offers the label-free and on-demand separation of different-sized micro-objects in moving droplets. The present method has potential uses such as in-droplet sample purification and enrichment.

References

Sep 5, 2002·Electrophoresis·Peter R C Gascoyne, Jody Vykoukal
May 15, 2004·Science·Lotien Richard HuangJames C Sturm
Aug 31, 2006·The Journal of the Acoustical Society of America·Jungwoo Lee, K Kirk Shung
Nov 7, 2006·Angewandte Chemie·Helen SongRustem F Ismagilov
May 16, 2007·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Charles N BaroudRégis Wunenburger
Jun 16, 2007·Analytical Chemistry·Filip PeterssonThomas Laurell
Jul 27, 2007·Lab on a Chip·Luis M FidalgoWilhelm T S Huck
Apr 16, 2010·Lab on a Chip·Irena Barbulovic-NadAaron R Wheeler
Jul 21, 2011·Biomicrofluidics·Byungwook AhnKwang W Oh
Feb 10, 2012·Lab on a Chip·Mira T GuoDavid A Weitz
Apr 19, 2012·Lab on a Chip·Wynter J DuncansonDavid A Weitz
Jun 2, 2012·Biomicrofluidics·G K Kurup, Amar S Basu
Mar 19, 2013·Analytical Chemistry·Thomas SchneiderDaniel T Chiu
Apr 6, 2013·Nature Protocols·Linas MazutisJohn A Heyman
Apr 30, 2013·Lab on a Chip·Sunil K AryaAbdur Rub Abdur Rahman
Jun 12, 2013·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Erfan KadivarMartin Brinkmann
Mar 29, 2014·Advanced Materials·Richie J ShiltonMarco Cecchini
Oct 15, 2014·Lab on a Chip·Ghulam DestgeerHyung Jin Sung
Dec 17, 2014·Lab on a Chip·Eric BrouzesHelmut H Strey
Dec 30, 2014·Lab on a Chip·Ilke AkartunaDavid A Weitz
Oct 1, 2015·Analytical Chemistry·Anna FornellMaria Tenje
May 24, 2016·Lab on a Chip·Tomasz S KaminskiPiotr Garstecki
Jul 21, 2016·Lab on a Chip·Jin Ho JungHyung Jin Sung
Nov 15, 2016·Lab on a Chip·Pingan Zhu, Liqiu Wang
Nov 29, 2016·Scientific Reports·V B VarmaR V Ramanujan
Mar 1, 2017·Lab on a Chip·Jinsoo ParkHyung Jin Sung
Mar 11, 2017·PloS One·Sida WangMark A Burns
May 30, 2017·Biosensors & Bioelectronics·Song-I HanArum Han

❮ Previous
Next ❯

Citations

Jun 2, 2020·Biomicrofluidics·Li-Qiang LiKe-Ji Yang
Aug 17, 2020·Biomedical Microdevices·Lujing SunLianqing Liu
Jan 1, 2021·Biomicrofluidics·Michael S GerltJürg Dual
Apr 7, 2021·Scientific Reports·Anna FornellMaria Tenje
Jul 17, 2021·Lab on a Chip·Kirill KolesnikDavid J Collins
Jan 18, 2020·Journal of the American Chemical Society·Sungu KimRobbyn K Anand

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.