In-droplet microparticle washing and enrichment using surface acoustic wave-driven acoustic radiation force

Lab on a Chip
Jinsoo ParkHyung Jin Sung

Abstract

Washing and enrichment of particles and cells are crucial sample preparation procedures in biomedical and biochemical assays. On-chip in-droplet microparticle washing and enrichment have been pursued but remained problematic due to technical difficulties, especially simultaneous and precise control over the droplet interface and in-droplet samples. Here, we have achieved a breakthrough in label-free, continuous, on-demand, in-droplet microparticle washing and enrichment using surface acoustic waves. When exposed to the acoustic field, the droplet and suspended particles experience acoustic radiation force arising from inhomogeneous wave scattering at the liquid/liquid and liquid/solid interfaces. Based on these acoustophoretic phenomena, we have demonstrated in-droplet microparticle washing and enrichment in an acoustofluidic device. We expect that the proposed acoustic method will offer new perspectives to sample washing and enrichment by performing the operation in microscale droplets.

References

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Jul 12, 2012·Lab on a Chip·Sz-Chin Steven LinTony Jun Huang
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Nov 6, 2014·Lab on a Chip·Sixing LiTony Jun Huang
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Apr 8, 2015·Proceedings of the National Academy of Sciences of the United States of America·Peng LiTony Jun Huang
Jul 4, 2015·Scientific Reports·Byung Hang HaHyung Jin Sung
Jul 15, 2015·Ultrasound in Medicine & Biology·Muhannad N FadhelMichael C Kolios
Oct 1, 2015·Analytical Chemistry·Anna FornellMaria Tenje
Oct 22, 2015·Analytical Chemistry·Byung Hang HaHyung Jin Sung
Apr 7, 2016·Analytical Chemistry·Byunghang HaHyung Jin Sung
May 18, 2016·Nature Communications·Per AugustssonJoel Voldman
Jul 21, 2016·Lab on a Chip·Jin Ho JungHyung Jin Sung
Dec 21, 2016·Lab on a Chip·Muhsincan SesenAdrian Neild
Mar 1, 2017·Lab on a Chip·Jinsoo ParkHyung Jin Sung
Mar 11, 2017·PloS One·Sida WangMark A Burns
May 30, 2017·Biosensors & Bioelectronics·Song-I HanArum Han
Jun 21, 2017·Lab on a Chip·Muhsincan SesenAdrian Neild
Nov 18, 2017·Analytical Chemistry·Raheel AhmadHyung Jin Sung

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Citations

Apr 10, 2019·Lab on a Chip·Steven R DoonanRyan C Bailey
Jan 1, 2021·Biomicrofluidics·Michael S GerltJürg Dual
Jun 3, 2021·Analytical Chemistry·Muhammad AfzalHyung Jin Sung
May 25, 2021·Biomicrofluidics·Zhenhua LiuMaria Tenje
Jul 1, 2021·Lab on a Chip·Xianming QinDong Sun
Nov 11, 2020·Analytical Chemistry·Simon F BerlandaPetra S Dittrich
May 23, 2020·Analytical Chemistry·Jason ZhouReza Nosrati
May 18, 2019·Analytical Chemistry·Muhsincan SesenAdrian Neild

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Methods Mentioned

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