In-host microevolution of Aspergillus fumigatus: A phenotypic and genotypic analysis

Fungal Genetics and Biology : FG & B
Eloise BallardAdilia Warris

Abstract

In order to survive, Aspergillus fumigatus must adapt to specific niche environments. Adaptation to the human host includes modifications facilitating persistent colonisation and the development of azole resistance. The aim of this study is to advance understanding of the genetic and physiological adaptation of A. fumigatus in patients during infection and treatment. Thirteen A. fumigatus strains were isolated from a single chronic granulomatous disease patient suffering from persistent and recurrent invasive aspergillosis over a period of 2 years. All strains had identical microsatellite genotypes and were considered isogenic. Whole genome comparisons identified 248 non-synonymous single nucleotide polymorphisms. These non-synonymous mutations have potential to play a role in in-host adaptation. The first 2 strains isolated were azole susceptible, whereas later isolates were itraconazole, voriconazole and/or posaconazole resistant. Growth assays in the presence and absence of various antifungal stressors highlighted minor changes in growth rate and stress resistance, with exception of one isolate showing a significant growth defect. Poor conidiation was observed in later isolates. In certain drug resistant isolates conidiation...Continue Reading

Citations

Jul 26, 2018·PloS One·Jan ZollWillem J G Melchers
Jan 16, 2020·Journal of Fungi·Mireille H van der TorreRiina Rautemaa-Richardson
Sep 25, 2019·Nature Microbiology·Caitlin H KowalskiRobert A Cramer
Jul 29, 2020·Applied Microbiology and Biotechnology·Zsuzsa SzabóIstván Pócsi
Sep 26, 2019·Frontiers in Microbiology·Fabiola Puértolas-BalintSilvia García-Cobos
May 23, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Ingrid Ludmila Rodrigues CruzRodrigo Almeida-Paes
Mar 19, 2019·Emerging Infectious Diseases·Tobias G P EngelPaul E Verweij
Sep 4, 2018·Frontiers in Microbiology·Takahiro Takazono, Koichi Izumikawa
Mar 9, 2021·Virulence·Marie-Fleur DurieuxFrançoise Botterel
Mar 7, 2021·Journal of Fungi·Mireille H van der TorreLilyann Novak-Frazer
Mar 10, 2019·Biochimica Et Biophysica Acta. Proteins and Proteomics·Brian C MonkMikhail V Keniya
May 27, 2021·Studies in Mycology·A ArastehfarM Hoenigl
Sep 8, 2021·Mycopathologia·Amelie P BrackinJohanna Rhodes

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

Qualimap
EnsemblFungi Effect Predictor
VCFtools
Muscle
SNPhylo
Bowtie2
Phylip
Integrated Genomics Viewer Tablet
FreeBayes
EnsemblFungi

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.